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Alterations of Thymic Epithelial Cells in Lipopolysaccharide-induced Neonatal Thymus Involution

摘要:

Background: Vascular endothelial growth factor (VEGF) in the thymus was mainly produced by the thymic epithelial cells (TECs), the predominant component of the thymic microenvironment.The progression of TECs and the roles of VEGF in the neonatal thymus during sepsis have not been reported.This study aimed to explore the alterations of TECs and VEGF level in the neonatal thymus involution and to explore the possible mechanisms at the cellular level.Methods: By establishing a model of clinical sepsis, the changes of TECs were measured by hematoxylin-eosin staining, confocal microscopy, and flow cytometry.Moreover, the levels of VEGF in serum and thymus were assessed based on enzyme-linked immunosorbent assay and Western blotting.Results: The number ofthymocytes and TECs was significantly decreased 24 h after lipopolysaccharide (LPS) challenge, (2.40 ± 0.46)× 107 vs.(3.93 ± 0.66)×107 and (1.16 ± 0.14)×105 vs.(2.20 ± 0.19)×105, P < 0.05, respectively.Cortical TECs and medullary TECs in the LPS-treated mice were decreased 1.5-fold and 3.9-fold, P < 0.05, respectively, lower than those in the controls.The number of thymic epithelial progenitors was also decreased.VEGF expression in TECs was down-regulated in a time-dependent manner.Conclusion: VEGF in thymic cells subsets might contribute to the development of TECs in neonatal sepsis.

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作者单位: Affiliated Mental Health Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430012, China [1] Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China [2]
期刊: 《中华医学杂志(英文版)》2016年129卷1期 59-65页 SCIMEDLINEISTICCSCDBP
栏目名称: Original Articles
DOI: 10.4103/0366-6999.172577
发布时间: 2016-02-26
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