• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
取消
高级检索

检索历史 清除

医学文献 >>
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
知识库 >>
  • 临床诊疗知识库
  • 中医药知识库
评价分析 >>
  • 机构
  • 作者
热搜词:
换一批

Identification of Circular RNAs as a Novel Biomarker for Ovarian Endometriosis

摘要:

Background:Endometriosis is a challenging disease with symptoms such as dysmenorrhea and infertility.However,its etiology is still vague and there is still no effective markers or treatment.This study aimed to profile the circular RNAs (circRNAs) expressed in eutopic endometrium from patients with ovarian endometriosis and explore potential clues to the pathogenesis of endometriosis,providing an evidence for clinical diagnosis and treatment.Methods:A total of 63 clinical samples,including control endometrium (n =22) and eutopic endometrium (n =41),were collected from Peking Union Medical College Hospital between May 1,2016,and December 31,2016.Of them,four samples in each group were used for circRNA microarray.Then,four upregulated circRNAs were screened out for quantitative real-time polymerase chain reaction (qRT-PCR)validation.After that,bioinformatics analysis was performed to predict miRNAs targeted by validated circRNAs and investigate the circRNA-miRNA-mRNA interactions.Results:Among 88 differentially expressed circRNAs,11 were upregulated and 77 were downregulated in eutopic endometrium of patients with endometriosis.qRT-PCR validation results for two upregulated circRNAs (circ_0004712 and circ_0002198) matched the microarray results.The area under the receiver operating characteristic curve of circ_0002198 for distinguishing ovarian endometriosis was 0.846 (95% confidence interval [CI]:0.752-0.939;P < 0.001) while that ofcirc_0004712 was 0.704 (95% CI:0.571-0.837;P =0.008).On the basis of target prediction,we depicted the molecular interactions between the identified circRNAs and their dominant target miRNAs,as well as constructed a circRNA-miRNA-mRNA network.Conclusions:This study provides evidence that circRNAs are differentially expressed between eutopic and normal endometrium,which suggests that circRNAs are candidate factors in the activation ofendometriosis.circ_0002198 and circ_0004712 may be potential novel biomarkers for the diagnosis of ovarian endometriosis.

更多
作者: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730, China [1]
作者单位: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730, China [1]
期刊: 《中华医学杂志(英文版)》2018年131卷5期 559-566页 SCIMEDLINEISTICCSCDBP
栏目名称: Original Articles
DOI: 10.4103/0366-6999.226070
发布时间: 2021-07-16
基金项目:
This study was supported by grants from the National Key R&D Program of China and National Natural Science Foundation of China
翻译满意度评价:
提交
  • 浏览:51
  • 下载:36

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

  • 客服热线:4000-115-888 转3 (周一至周五:8:00至17:00)

  • |
  • 客服邮箱:yiyao@wanfangdata.com.cn

  • 违法和不良信息举报电话:4000-115-888,举报邮箱:problem@wanfangdata.com.cn,举报专区

官方微信
万方医学小程序
new翻译 充值 订阅 收藏 移动端

官方微信

万方医学小程序

使用
帮助
Alternate Text
调查问卷