检索历史 清除

目的　研究第2代人发角蛋白（human hair keratin Ⅱ, HHK-Ⅱ）人工腱植入后不同时期转化生长因子-β1，2，3（TGF-β1，2，3） mRNA 的表达及泛素(ubiquitin)的分布。　方法　选用4只新西兰兔制作动物模型，用原位杂交 (in situ hybridization, ISH) 的方法研究HHK-Ⅱ人工腱植入后不同时期TGF-β1，2，3 mRNA 的表达；用免疫组化法研究泛素的分布。　结果　TGF-β1，2，3 mRNA用原位杂交方法均能检测到，但它们分布的位置不同。TGF-β1 mRNA仅存在于人工腱周围新生的腱成纤维细胞质中。TGF-β2 mRNA在新生腱组织中广泛存在，在半成熟的腱组织中也有一定程度的表达；而TGF-β3 mRNA在新生腱组织和较成熟腱组织中表达量很低。在正常自体腱组织中，泛素呈低表达，且均匀分布；HHK-Ⅱ人工腱植入后3周，人工腱之间及其周围的新生腱组织中泛素表现为强阳性；植入后6周和9周的泛素为弱阳性，其中9周的泛素接近正常腱组织。即在人工腱降解期泛素表现为强阳性，自体腱形成期及之后的时期泛素的表达明显下调。　结论　在肌腱修复时，TGF-β1，2可能起主要作用，TGF-β3在肌腱修复过程中起协同作用。人工腱角蛋白的降解可能主要由泛素承担；人工腱周围新生组织中不正常蛋白的清除除有溶酶体参与外，泛素也起主要作用。

Objective　To study the expression of TGF-β1,2,3 mRNA and distribution of ubiquitin after artificial tendon HHK-Ⅱ implantation. 　Methods　Four New Zealand rabbits were used as animal models to study the mRNA expression of TGF-β1,2,3 by in situ hybridization and to detect the distribution of ubiquitin by immunohistochemistry. 　Results　TGF-β1, 2, 3 mRNA could be detected, but their distribution was different. Of TGF-β1 mRNA only was located in newly generated fibroblasts around HHK-Ⅱ, of TGF-β2 mRNA displayed in large area of tendon tissues and TGF-β3 mRNA expressed lowly. Ubiquitin was lowly expressed in normal tendon tissues and dispersed evenly. Ubiquitin had high positiveness in the newly generated tissues between HHK-Ⅱ and around it 3 weeks after operation. Ubiquitin was decreased distinctly 6 and 9 weeks after operation. Ubiquitin, 9 weeks after implantation of HHK-Ⅱ, was decreased nearly to the same as that in normal tendon tissues. 　Conclusions　TGF-β1,2 may play a critical role in tendon healing, TGF-β3 cooperates with them. Degeneration of HHK-Ⅱ keratin may mainly be undertaken by ubiquitin. Lysosome can clear the abnormal protein in newly generated tissues near HHK-Ⅱ, ubuquitin also takes part in this process.