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大鼠轴索损伤后视网膜神经节细胞和少突胶质细胞内Tau蛋白的表达

Expression changes of Tau protein in retinal ganglion cells and oligodendrocytes following axonal injury in rats

摘要:

目的 观察大鼠轴索损伤后视网膜神经节细胞(retinal ganglion cells,RGCs)及少突胶质细胞(oligodendrocytes,OLs)内Tau蛋白表达的变化,探讨Tau蛋白与轴索损伤后病理变化的关系. 方法 光镜下观察正常对照组、单纯视神经牵拉伤组、单纯热应激处理组和热应激预处理牵拉伤组大鼠视神经、RGCs、OLs的形态学变化,免疫组化染色检测各组大鼠RGCs及OLs中Tau蛋白的表达情况. 结果 牵拉伤后视神经轴索、RGCs及OLs的形态发生明显的病理变化,热应激预处理再致伤后上述病理改变有显著改善.单纯牵拉伤可使RGCs及OLs中Tan蛋白的表达增加,单纯热应激处理后Tau蛋白的表达不受影响,热应激预处理后再致伤使Tau蛋白的表达明显减弱. 结论 神经元及神经胶质细胞共同参与了轴索损伤后的病理过程.Tau蛋白表达变化可能与迟发性轴索断裂及迟发性神经元凋亡有关.热应激能使Tau蛋白表达减弱和延后,减轻细胞骨架的损害.

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Objective To investigate expression changes of Tau protein in retinal ganglion cells (RGCs) and oligodendrocytes (Ols) after stretch injury in rats and explore the relationship of Tau protein with pathological changes after axonal injury. Methods Morphological changes of optic nerves, RGCs and OLs after stretch injury were examined under light microscope in control group, stretch only group, heat stress only group and heat stress pretreatment plus stretch group. The expressions of Tau protein in RGCs and OLs after heat stress and/or stretch injury were observed by using immunohistechemical stai-ning. Results Pathological changes of axons, RGCs and OLs were identified morphologically or quan-titatively after stretch injury to the optic nerves, which was significantly ameliorated through pretreatment with heat stress plus stretch injury. The expressions of Tau protein in RGCs and OLs were increased in stretch only group. There was no significant expression change of Tau protein in heat stress only group. Expression of Tan protein was obviously decreased in heat stress pretreatment plus stretch group. Con-clusions Both neurons and glial cells are involved in pathological process after axonal injury. The ex-pression changes of Tau protein are probably related to delayed axotomy and neuron apoptosis. Heat stress can relieve the impairment of cystoskeleton through decreasing and delaying the expression of Tau protein.

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