大鼠肾损伤早期三七总皂苷对肾皮质小管细胞Bax的影响
Effect of panax notoginseng saponins on renal cortical tubule cell Bax in early stage after renal trauma in rats
目的 研究三七总皂苷(panax notoginseng saponins,PNS)对肾损伤早期肾皮质小管细胞凋亡及凋亡调控基因的影响,探讨PNS对肾损伤的保护机制. 方法 Wistar大鼠78只按随机数字表法分为创伤组(36只)、创伤给药组(给药组,36只)、正常对照组(对照组,6只);给药组采用腹腔注射PNS 70 mg/kg,创伤组及对照组给予腹腔注射等剂量的等渗盐水;创伤组和给药组又分为6个亚组:即打击后1,6,12,24,36,48 h组,每组6只.在各损伤时相点提取肾脏标本,免疫组化染色及原位杂交组织化学技术检测Bax在肾脏皮质细胞中的表达,观察阳性表达指标在各组之间的差异以及随时间变化的规律性. 结果 创伤组肾皮质浅层促凋亡基因Bax mRNA转录在12h增加,而肾皮质深层Bax mRNA转录则在1h增加;促凋亡因子Bax蛋白表达在肾皮质浅层于36 h前未明显下降,皮质深层于12 h前明显下降.给药组肾皮质全层给药后即刻减少促凋亡基因Bax mRNA转录并持续至48 h;肾皮质各层促凋亡因子Bax蛋白表达在各时相点均呈单向性下降直至48 h. 结论 PNS下调Bax mRNA的转录与蛋白的表达,从而起到抑制细胞凋亡的作用.
更多Objective To measure the effect of panax notoginseng saponins (PNS) on renal cortical tubule cell apoptosis and apoptosis-related genes in early stage after renal trauma and to investigate the protective mechanism of PNS for renal trauma.Methods Seventy-eight Wistar rats were divided into trauma group (n =36),trauma + treatment group (treatment group,n =36),normal control group (control group,n =6) according to the random number table.In treatment group,rats received intraperitoneal administration of PNS (70 mg/kg).Instead,substitute of an equal dose of isotonic saline was used for the rats in trauma and normal control groups.Trauma and treatment groups were subdivided at 1,6,12,24,36 and 48 hours posttrauma,with 6 rats per group.The kidney specimens were extracted at each time point to detect Bax expression in the cortex with immunohistochemistry and in situ hybridization histochemistry.Moreover,the positive expression of Bax was compared among groups and its variation regularity with time were detected.Results In trauma group,mRNA transcription of pro-apoptosis gene Bax increased at 12 hours in the superficial cortex,but increased at 1 hour in deep cortex; protein expression of pro-apoptosis factor Bax showed no apparent reduction within 36 hours in the superficial cortex,but evident decrease within 12 hours in the deep cortex.In treatment group,mRNA transcription of pro-apoptosis gene Bax decreased immediately after treatment in the renal cortex and lasted until 48 h; protein expression of pro-apoptosis factor Bax showed unidirectional reduction until 48 h in the renal cortex.Conclusion PNS inhibits cell apoptosis by down-regulating the mRNA and protein expression of Bax.
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