FK506缓释剂促进周围神经轴浆运输功能恢复的实验研究
Functional recovery of axoplasmic transport of regenerative peripheral nerve promoted by FK506
目的 探讨FK506缓释剂对周围神经轴浆运输功能恢复的促进作用.方法 取48只SD大鼠,应用自行研制的梭形双通道桥接管桥接长10 mm的大鼠坐骨神经缺损.根据梭形管的2支管内加入的药物不同将动物随机均分为A组(24只2支管内均加入几丁糖凝胶)、B组[24只,一侧支管内注入几丁糖和FK506(B1组),另一侧支管内注入几丁糖和等渗生理盐水(B2组)].术后12、16周对再生神经行辣根过氧化物酶和核黄逆行示踪,术后16周行再生神经传导速度测定和透射电镜观察.结果 术后12、16周,透射电镜观察到A组2支管内再生纤维无明显差异,B1组较B2组再生纤维数量多,排列整齐,粗细均匀;辣根过氧化物酶和核黄显示:与B2组相比,B1组再生神经示踪后,脊髓内被标记的阳性神经元数目多,分布稠密;B1组神经传导速度为35.62 m/s +3.23 m/s,B2组为22.62 m/s±2.12 m/s,两组比较差异有统计学意义(P<0.05).结论 FK506缓释剂对周围神经再生纤维形态结构和功能的恢复具有促进作用.
更多Objective To explore the effect of FK506 (tacrolimus) on functional recovery of axoplasmic transport of the regenerative peripheral nerve.Methods Forty-eight Sprague-Dawley rats with 10 mm gap of sciatic nerve were randomly divided into 2 even groups.The nerve defects were bridged with our self-designed double-channel nerve conduits of fusiform shape.In group A,100 μL of chitin for medical use was injected into each of the 2 channels of the conduit; in group B,after 100 μL of chitin was injected into each of the 2 channels,one channel of the conduit was injected with 10 μL of FK506 (subgroup B1 ) and the other with 10 μL of isotonic saline (subgroup B2).At 12 and 16 weeks after operation,we evaluated axoplasmic transport of the regenerative nerve in the 2 channels with horseradish peroxidase(HRP) and nuclear yellow(NY) observations.At 16 weeks regenerative nerve conduction velocity(NCV) was measured and ultrastructure of the regenerative nerve was also observed with transmission electron microscopy(TEM).Results At 12 and 16 weeks after operation,TEM showed no significant difference in the regenerative nerve fibers between the 2 channels in group A.In group B,more well-arranged and even regenerative fibers were observed in subgroup B1 than in subgroup B2.The HRP and NY tracing showed much more positive neurons densely distributing in the spine cord in subgroup B1 than in subgroup B2.The NCV was 35.62 m/s +3.23 m/s in subgroup B1,significantly faster than that (22.62 m/s ±+ 2.12 m/s) in subgroup B2( P < O.05).Conclusion Functional recovery of axoplasmic transport of the regenerative nerve fibers can be significantly promoted by FK506.
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