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目的 筛选创伤性深静脉血栓形成(DVT)消退关键基因,探讨这些基因在创伤性DVT消退中的意义. 方法 取70只SD大鼠,分为正常对照组(A组,10只)和模型组(60只),通过定量打击方法建立创伤性DVT动物模型.模型组再筛选出2个亚组:血栓形成组(B组,10只)和血栓消退组(C组,10只),采用Genechip Rat Genome 430 2.0基因表达谱芯片检测DVT发生、发展过程中创伤局部静脉内基因差异表达的变化,应用时间序列分析方法进行分析,筛选影响血栓消退实验表型变化的关键基因. 结果 创伤性DVT消退关键基因主要有胶原Ⅺ-α1(COL11-α1)、葡萄糖醛酸酶-B(GUs-β)、基质金属肽酶-12(MMP-12)、成纤维细胞激活蛋白(FAP)、C1q和肿瘤坏死因子相关蛋白-3(C1QTNF3_PREDICTED)、酸性磷酸酶-5(ACP-5)、GTP酶激活因子12 (TBC1D12_PREDICTED)、钙蛋白酶-6(CAPN-6)等基因.A、B、C组COL11-α1基因丰度表达值分别为0、2.5、1.2,GUS-β基因丰度表达值分别为0、1.5、0.7,MMP-12基因丰度表达值分别为0、3.9、1.6,FAP基因丰度表达值分别为0、1.8、0.5,C1QTNF3_PREDICTED基因丰度表达值分别为0、5.7、3.5,ACP-5基因丰度表达值分别为0、1.2、0.8,TBC1D12_PREDICTED基因丰度表达值分别为0、1.7、0.8,CAPN-6基因丰度表达值分别为0、4.5、2.9.结论 在创伤性DVT演化过程中,COL11-α1、GUS-β、MMP-12、FAP、C1QTNF3_PREDICTED、ACP-5、TBC1D12_PREDICTED、CAPN-6等基因与血栓消退关系密切,是促进血栓消退的关键基因.

Objective To screen the key genes for thrombi resolution in a rat model of trauma deep vein thrombosis (DVT). Methods Seventy Sprague Dawley rats were divided into a normal control group (group A,10 rats) and a model group (60 rats).The traumatic DVT model was established by quantitative beating on the bilateral posterior limbs.From the model group a thrombosis group (group B,10 rats) and a thrombosis-resolution group (group C,10 rats) were screened.The Genechip Rat genome 430 2.0 was used to detect changes in gene expressions on difference phases of DVT after trauma.Time series analysis was used to screen the key genes that affected the phenotype changes associated with thrombi resolution. Results COL11-α1,GUS-β,MMP12,FAP,C1QTNF3_PREDICTED,ACP-5,TBC1D12_PREDICTED and CAPN-6 were screened as the key genes for thrombi resolution in traumatic DVT.In groups A,B and C,the abundance expressions of COL11-α1 were respectively 0,2.5 and 1.2; those of GUS-β were respectively 0,1.5 and 0.7; those of MMP-12 were respectively 0,3.9 and 1.6; those of FAP were respectively 0,1.8 and 0.5; those of C1QTNF3_PREDICTED were respectively 0,5.7 and 3.5; those of ACP-5 were respectively 0,1.2 and 0.8; those of TBC1D12_PREDICTED were respectively 0,1.7 and 0.8.; those of CAPN-6 were respectively 0,4.5 and 2.9.These genes had the same expression mode and coincided with the biological phenotype of thrombi. Conclusion In the process of traumatic DVT,as COL11-α1,GUS-β,MMP-12,FAP,C1QTNF3_PREDICTED,ACP-5,TBC1D12_PREDICTED and CAPN-6,have an intimate relation with the resolution of thrombi,they may be the key genes that promote thrombi resolution.