摘要目的 分析常见分枝杆菌不同株之间的因型,为临床分离株的鉴定提供参考序列.方法 用16S-23S rRNA转录间隔区(internal transcribed spacer,ITS)序列分析法对94株由德国微生物和细胞保藏中心(DSMZ)引进的常见分枝杆菌进行种内不同株的基因型分析,并分别与12株国际标准株对比.通过种内不同株间同源性序列比对,绘制16S-23S rRNA ITS序列聚类分析树状谱,使用DNAStar的MegAlign软件计算株间相似性百分比.结果 成功完成上述共106株菌株的16S-23SrRNA ITS测序,发现除胞内分枝杆菌(4株)、鸟分枝杆菌(4株)、海分枝杆菌(6株)和马尔摩分枝杆菌(2株)各有一个基因型且与标准株相同外,其他分枝杆菌均可分为数个不同基因型.结论 16S-23S rRNA ITS序列分析可以根据种内各株的不同基因型将分枝杆菌鉴定到株,是分枝杆菌基因型分析的可靠方法.

abstractsObjective To analyze the genotypes of intraspecies of common mycobacterin.Methods The genotypes of 94 strains of mycobacteria from DSMZ,as compared with 12 reference strains.were studied by 16S-23S rRNA intemal transcription space (ITS) sequence analysis.Results The sequencing of 16S-23S rRNA ITS of the 106 strains of common mycobacteria were completed.All the mycobacteria could be discriminated to several genotypes except 4 M.intraeeUulare.4 M.avium,6 M.marinum and 2 M.malmoense which were identical to their reference strains.Conclusions 16S-23S rRNA ITS sequence analysis iS a reliable method to discriminate mycobacteria in interspecies even in intraspecies.