胰岛素样生长因子-Ⅰ对雌性大鼠尿道括约肌损伤后修复的影响
Influence of periurethral injection of insulin-like growth factor-Ⅰ on the expression of IGF-Ⅰ and IGF-Ⅱ mRNA following urethral sphincter muscle injury in female rats
目的 探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)对雌性大鼠尿道括约肌损伤后修复的影响.方法 雌性未育SD大鼠55只分3组,治疗组(n=25)采用阴道扩张模拟产伤方法建立尿道括约肌损伤模型,尿道中段注射IGF-Ⅰ 1.0 μg(浓度0.1μg/μl);生理盐水对照组(n=25)尿道括约肌损伤模型建立后中段尿道注射无菌生理盐水10μl;正常对照组(n=5)无损伤不做任何处理.RT-PCR法检测治疗组、生理盐水对照组损伤后2、4、6、8、14 d时及正常对照组大鼠尿道括约肌组织IGF-Ⅰ和Ⅱ mRNA的相对表达量,比较治疗组和生理盐水对照组尿道括约肌损伤后不同时间IGF-Ⅰ和ⅡmRNA的表达差异. 结果 尿道括约肌组织伤后2、4、6、8、14 d,生理盐水对照组大鼠IGF-Ⅰ mRNA表达量分别为未测出、0.58±0.15、1.73±0.31、2.30±0.29、0.46±0.06,治疗组分别为0.69±0.21、1.45±0.17、2.25±0.45、2.90±0.49、1.92±0.31,与生理盐水对照组比较第4、8、14天表达差异有统计学意义(P<0.05);生理盐水对照组尿道括约肌组织伤后4、6、8 d IGF-Ⅱ mRNA表达量分别为0.42±0.14、1.51±0.59、1.31±1.04,治疗组4、6、8、14d表达量分别为1.04±0.23、1.94±0.29、1.75±0.41、0.81±0.15,与生理盐水对照组比较第4天差异有统计学意义(P<0.01).正常对照组尿道组织无IGF-Ⅰ、Ⅱ mRNA表达. 结论 局部注射IGF-Ⅰ能促进尿道括约肌损伤局部内源性IGF-Ⅰ和ⅡmRNA表达,IGF-Ⅰ对尿道括约肌损伤所致压力性尿失禁可能具有潜在的治疗作用.
更多Objective To investigate the effect of periurethral injection of insulin-like growth factor(IGF)-Ⅰ on the expression of IGF-Ⅰ and IGF-Ⅱ mRNA during regeneration period following urethral sphincter muscle injury in female rats. Methods Model of urethral sphincter muscle injury was made in female virgin SD rats (n=50) by intravaginal balloon inflation. Then the rats were divid-ed randomly into treatment group (n= 25) and control group (n= 25), treatment group accepted peri-urethral injection of 1.0μg human IGF-Ⅱ to the middle urethral muscle, control group accepted nor-mal saline injection. Five rats in each group were sacrificed at 2, 4, 6, 8, 14 day respectively and the whole urethra specimens were processed for RT-PCR to detect the expression of IGF-Ⅰ ,Ⅱ mRNA. A normal control group (n = 5) was set without intravaginal balloon inflation and injection. Results The expression of IGF-Ⅰ mRNA in control group increased at day 4, 6, 8, 14, the IGF-Ⅰ/β-actin ra-tios were 0. 58±0.15, 1.73±0.31, 2.30±0.29, 0. 46±0. 06. The expression of IGF-Ⅰ mRNA in treatment group increased at all time points, as 0. 69±0.21, 1.45±0.17, 2.25±0.45, 2.90±0.49, 1.92±0. 31. The difference was significant on day 4, 14(P<0.01), and day 8 (P<0.05) compared with the control group. The expression of IGF-Ⅱ mRNA in control group increased at day 4, 6, 8, as 0.42±0. 14, 1.51±0. 59, 1.31±1.04. The expression of IGF-Ⅱ mRNA in treatment group in-creased at day 4, 6, 8, 14, as 1.04±0.23, 1.94±0.29, 1.75±0.41, 0. 81±0.15. The significant difference was noted on day 4 (P<0. 01)compared with the control group. No expression of IGF-Ⅰand Ⅱ mRNA in the normal control group. Conclusions The expression of endogenous IGF-Ⅰ and IGF-Ⅱ mRNA was up-regulated by periurethral injection of IGF-Ⅰ during regeneration period follow-ing urethral sphincter muscle injury in female rat. Our findings suggest that IGF-Ⅰ facilitates the re-generation of the urethral muscles and may play a role in treatment of stress urinary incontinence in-duced by urethral sphincter muscle dysfunction.
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