• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
取消
高级检索

检索历史 清除

医学文献 >>
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
知识库 >>
  • 临床诊疗知识库
  • 中医药知识库
评价分析 >>
  • 机构
  • 作者
热搜词:
换一批

草酸钙结石模型大鼠肾脏miRNA差异表达谱的分析研究

Analysis of the MicroRNA expression profiles in the kidney of calcium oxalate stones rats

摘要:

目的 比较草酸钙结石模型大鼠与正常大鼠肾组织小分子RNA(microRNA,miRNA)表达谱的差异,初步分析筛选出的差异miRNA的功能.方法 2015年9-12月,将16只6~8周龄雄性SD大鼠随机分为两组,每组8只.正常组每天自由饮水.造模组使用乙二醇和氯化铵诱导法建立大鼠肾草酸钙结石模型.28 d后应用微阵列芯片筛选正常组和造模组肾组织标本中差异表达(表达量>2倍)的miRNA,对差异表达的miRNA进行靶基因预测,并运用Gene Ontology数据库和KEGG信号通路数据库富集靶基因以初步分析其功能.结果 偏光显微镜下观察肾组织切片,正常组未见草酸钙晶体,造模组可见大量草酸钙晶体.与正常组相比,造模组38个miRNA表达有显著差异,其中上调18个,下调20个;基因本体分析筛选出有显著差异的信使RNA(mRNA)共2728个,其中上调1350个,下调1378个,差异均有统计学意义(P<0.05).Gene Ontology分析结果显示差异性表达的miRNA与钙离子转运、炎症、损伤、氧化应激、黏附等生物学过程相关.KEGG信号通路分析结果显示这些miRNA参与趋化因子信号通路、细胞因子-细胞因子受体相互作用等与炎症反应相关的信号通路.结论 草酸钙结石大鼠与正常大鼠肾组织的miRNA表达谱存在明显差异,这些差异表达的miRNA可能与草酸钙结石形成过程中的钙离子转运、炎症反应、细胞损伤及细胞黏附等相关.

更多
abstracts:

Objective To compare the miRNA expression profiles in kidney tissues of normal and calcium oxalate stone rats,and to explore the roles of different miRNAs in the regulation of kidney stone formation.Methods Sixteen 6-8 weeks old SD rats were divided into two groups,including the normal group and the model group randomly,between Sep 2015 and Dec 2015.The normal group was permitted to drink water freely;whereas the model of calcium oxalate stone group was established by freely drinking water with 1% ethylene glycol and 1% ammonium chloride.28 days after molding,miRNA microarray and mRNA microarray were applied to evaluate the miRNA expressions in the kidney tissues of both normal and calcium oxalate stone rats.Target prediction,Gene Ontology (GO) analysis,Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were applied to predict the potential roles of miRNAs in the biological processes of stone formation.Results There were many crystals in calcium oxalate stone rat's kidney tissue sections whereas no crystal was detected in normal group.38 miRNAs were differentially expressed with a more than 2-fold changing (P < 0.05) between normal and calcium oxalate stone rats.Among those miRNAs,18 miRNAs were up-regulated,while 20 miRNAs were down-regulated.2728 mRNAs were differentially expressed with a more than 2-fold change (P < 0.05) between the two groups.Among them,1 350 mRNAs were up-regulated and 1 378 were down-regulated.Gene Ontology analysis suggested that those differentially expressed miRNAs targeted many genes which were related to the function of calcium ion transport,injury,inflammatory response,oxidative stress and cell adhesion.KEGG pathway analysis suggested that those miRNAs were involved in inflammation-related pathway including cytokine-cytokine receptor interaction and chemokine signaling pathway.Conclusions There were significantly differences in miRNA expression profiles between normal and calcium oxalate stone rats,and these differentially expressed miRNAs might be associated with the function of regulation of calcium ion transport,inflammatory response,response to wounding and cell adhesion in the biological processes of calcium oxalate stone formation.

More
作者: 陈康 [1] 蓝创歆 [1] 杨东 [1] 蔡超 [1] 段小鹿 [1] 徐绍洪 [1] 刘旸 [1] 陈东 [1] 麦新 [1]
期刊: 《中华泌尿外科杂志》2016年37卷5期 380-384页 ISTICPKUCSCD
栏目名称: 基础研究
DOI: 10.3760/cma.j.issn.1000-6702.2016.05.016
发布时间: 2016-07-28
基金项目:
国家自然科学基金 广州市教育局一般引导项目(2012C055,1201410963)National Natural Science Foundation of China General Guide Project of Guangzhou Bureau of Education
  • 浏览:244
  • 下载:100

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

扩展文献

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

  • 客服热线:4000-115-888 转3 (周一至周五:8:00至17:00)

  • |
  • 客服邮箱:yiyao@wanfangdata.com.cn

  • 违法和不良信息举报电话:4000-115-888,举报邮箱:problem@wanfangdata.com.cn,举报专区

官方微信
万方医学小程序
new翻译 充值 订阅 收藏 移动端

官方微信

万方医学小程序

使用
帮助
Alternate Text
调查问卷