• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
取消
高级检索

检索历史 清除

医学文献 >>
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
知识库 >>
  • 临床诊疗知识库
  • 中医药知识库
评价分析 >>
  • 机构
  • 作者
热搜词:
换一批

核苷酸结合寡聚化结构域样受体3炎症小体在草酸钙结石肾组织中的表达及意义

Expression of the nod-like receptor protein 3 inflammasome in calcium oxalate stone kidney

摘要:

目的 探讨核苷酸结合寡聚化结构域样受体3(nod-like receptor protein 3,NLRP3)炎症小体在草酸钙结石患者及大鼠模型肾组织中的表达及其临床意义.方法 收集2008年1月至2014年12月因肾结石行肾切除的肾组织标本20例为实验组,同期因肾细胞癌行根治性肾切除术标本中距肿瘤>2 cm且经病理证实为正常肾组织20例为对照组,应用免疫组化染色分别检测NLRP3、半胱氨酸天冬氨酸蛋白酶1(Caspase-1)及白细胞介素-1β(IL-1β)的表达水平.动物实验:将14只雄性SD大鼠随机分为实验组和对照组,每组7只;实验组采用乙二醇饮水法诱导建立草酸钙结石大鼠模型,对照组予蒸馏水饲养;饲养6周后留取大鼠的肾组织,通过HE及Pizzolato染色法观察实验组大鼠肾组织内结石形成情况,通过蛋白质印迹法和RT-PCR检测两组大鼠肾脏组织中NLRP3、Caspase-1及IL-1β的蛋白表达水平和mRNA表达水平.结果 NLRP3、Caspase-1及IL-1β在草酸钙结石患者的肾组织中呈强阳性表达,而在正常肾组织中呈弱阳性表达.动物实验结果显示实验组均成功构建草酸钙结石大鼠模型,在大鼠肾小管管腔内可见结石晶体形成,对照组肾组织内未见结石晶体.蛋白质印迹法检测结果显示,实验组NLRP3、Caspase-1及IL-1β的蛋白表达量分别为1.40±0.13、1.38 ±0.13、1.35±0.07,对照组分别为1.00±0.09、1.00±0.02、1.00±0.01,两组比较差异均有统计学意义(均P<0.05).RT-PCR检测结果显示实验组NLRP3、Caspase-1及IL-1β的mRNA表达量分别为1.00±0.15、1.29±0.27、3.46±0.72,对照组分别为0.44±0.08、1.00±0.12、1.00±0.15,两组比较差异均有统计学意义(均P <0.05).结论 草酸钙结石患者及大鼠模型肾组织中存在NLRP3炎症小体的过表达,NLRP3炎症小体为研究草酸钙结石形成的分子机制提供了新的思路.

更多
abstracts:

Objective To explore the expression level and significance of the nod-like receptor protein 3 (NLRP3) inflammasome in renal tissue with calcium oxalate stone.Methods 20 kidney specimens were collected as the experimental group from patients with calcium oxalate stone who underwent nephrectomy because of stones in our hospital between January 2008 and December 2014;another 20 renal specimens were get as the control group from patients with renal carcinoma,the renal tissues were obtained 2cm far from the tumor and proved as normal tissue.Immunohistochemical detection was carried out to analyze the expression level of NLRP3,Caspase-1,and IL-1β in the 40 renal samples.Animal experiment:fourteen male SD rats were randomly divided into calcium oxalate stone group and control group.For calcium oxalate stone group we established an ethylene glycol method induced hyperoxaluric rat model featured by crystalline material within tubule lumens;for control group normal feeding was performed.After 6 weeks,all rats were sacrificed,and the kidneys were harvested for further experiments.HE staining and Pizzolato staining were used to detect calcium oxalate crystals within tubule lumens.Western boltting and RT-PCR was applied to detect protein level and mRNA quantity of NLRP3,Caspase-1,and IL-1β from tissue lysates in rat model.Results In renal tissue samples obtained from patients with calcium oxalate stone disease,we demonstrated that the expression level of NLRP3,Caspase-1,and IL-1β were above to the normal renal tissue samples.We established a hyperoxaluric rat model character with crystalline material within tubule lumens examined by renal histology with HE staining and Pizzolato staining.And we detected that the protein and mRNA levels of NLRP3,Caspase-1 and IL-1β were remarkably increased in the lysates from the hyperoxaluric rat model (P < 0.05).Conclusions The NLRP3 inflammasome has overexpression in the renal tissue of patients with calcium oxalate stone as well as in the renal tissue of hyperoxaluric rat,and it provides a new thought to reveal the formation of calcium oxalate stone.

More
作者: 齐士勇 [1] 何振 [1] 姜行康 [1] 杨雄 [1] 张昌文 [1] 张志宏 [1] 徐勇 [1]
期刊: 《中华泌尿外科杂志》2016年37卷12期 940-944页 ISTICPKUCSCD
栏目名称: 基础研究
DOI: 10.3760/cma.j.issn.1000-6702.2016.12.016
发布时间: 2017-02-13
基金项目:
天津市卫生局科技基金项目(2015KZ102)Science and Technology Fund of Tianjin Health and Family Planning Commission
  • 浏览:139
  • 下载:132

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

扩展文献

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

  • 客服热线:4000-115-888 转3 (周一至周五:8:00至17:00)

  • |
  • 客服邮箱:yiyao@wanfangdata.com.cn

  • 违法和不良信息举报电话:4000-115-888,举报邮箱:problem@wanfangdata.com.cn,举报专区

官方微信
万方医学小程序
new翻译 充值 订阅 收藏 移动端

官方微信

万方医学小程序

使用
帮助
Alternate Text
调查问卷