摘要目的 定量观察治疗量环孢素A(CsA)对大鼠肝脏超微结构的影响.方法 SD大鼠24只,分为3组,每组8只.(1)对照组:口服橄榄油.(2)小剂量CsA组:口服CsA 25 mg·kg-1·d-1.(3)大剂量CsA组:口服CsA 50 mg·kg-1·d-1.用药2周后取肝组织制备超薄切片,电镜摄片.立体定量分析指标:(1)体积密度(Vv).(2)面积密度(Sv).(3)数密度(Nv).(4)比表面(δ).结果 CsA使肝细胞内质网Vv、Sv增加,δ值降低;微体Vv、Nv降低;高尔基体Vv降低;糖原减少;脂滴Vv增加.CsA对线粒体、溶酶体各项测定值无明显影响.结论 治疗量CsA可引起大鼠肝脏超微结构改变.

abstractsObjective To study the effect of CsA in a therapeutic dose on the hepatic ultrastructure in rats.Methods Twenty-four SD rats were divided into 3 groups.The control group was orally administered olive oil,and the other 2 groups were orally given CsA 25 mg/kg and 50 mg/kg daily respectively.After 2 weeks.the liver samples were taken for ultrathin sections.The stereological method was used to quantify some hepatic ultrastructural parameters including volume density (Vv),surface density(Sv),numerical density(Nv) and specific surface(δ).Results CsA caused an increases of Vv and Sv and a decrease of δ in endoplasmic reticulum;a decreases of Vv and Nv in microbody;decreases of Vv in Golgi complex and glycogen inclusions;an increases of Vv and Nv in lipid inclusions.Conclusion CsA in a therapeutic dose could cause the change of hepatic ultrastructure of rat.