昆明市2014-2015年轮状病毒分子流行病学特征
Rotavirus A pathogen surveillance and gene typing analysis in Kunming city of Yunnan province from July 2014 to June 2015
目的 分析2014年7月到2015年6月云南省昆明市全年龄组腹泻人群A组轮状病毒的感染状况及病原学特征,为A组轮状病毒感染的监测、防控及暴发病例的调查及疫苗研发提供基础数据和方法学参考.方法 采集云南省4个监测哨点医院全年龄组腹泻人群的粪便样本1 121份、非腹泻人群的粪便319份.提取样本RNA,逆转录聚合酶链反应(RT-PCR)鉴定A组轮状病毒阳性,对于阳性样本,进一步进行半巢式多重RT-PCR法进行G/P基因分型.结果 1 121份腹泻样本共检出A组轮状病毒244份,总阳性率21.8%.G基因分型:G9(66.4%,n=156)是最主要的基因型,其次是G3(18.7%,n=44)、G1(8.9%,n=21)和G8(1.7%,n=4),P基因分型:P[8](92.8%,n=218)是最主要的基因型,其次是P[4](4.7%,n=11).在G/P基因型方面,G9P[8](57.0%,n=134)是最主要基因型,其次是G3P[8](18.3%,n=43)和G1P[8](8.9%,n=21).轮状病毒流行有明显的季节分布特征(x2=46.3,P<0.001),流行高峰为冬季(31.2%);G9P[8](x2=27.3,P<0.001)、G3P[8](x2=10.2,P<0.042)和G1P[8](x2=8.2,P<0.039)的流行也都有明显的季节趋势,也都集中在冬季.<5岁人群中,阳性率在腹泻病例中高于非腹泻病例的基因型有G9P[8](14.9%,2.9%, x2=18.1,P<0.001)和G3P[8](4.4%,0.5%, x2=5.6,P<0.018). 结论 云南省腹泻人群中存在较高的A组轮状病毒感染率,病原体可分为多种基因型别,G9P[8]为优势型别.
更多Objective To analyze the infectious status and etiological characteristics of rotavirus A (RVA) from children less than five years old in the Kunming city of Yunnan province from July 2014 to June 2015, to provide basic data and methodological references for the disease surveillance, and controling outbreak cases investigations as well as vaccine research and development.Methods Stool samples were collected from 1 121 diarrhea cases and 319 healthy controls in four sentinel hospitals and transported to laboratory.Two-step reverse transcriptional polymerase chain reaction (RT-PCR) was used to detect the presence of RVA in each stool specimen.Suspicious positive specimens of gel electrophoresis was further sequenced to make definite diagnosis of RVA infection.Each RVA positive stool specimen was confirmed with sequencing which was conducted the semi-nested multiplex RT-PCR to detect six G genotypes and six P genotypes.Results Total 244 samples from the 1 212 diarrhea patients were detected positive for RVA and the total positive rate was 21.8%.G typing of group A rotavirus mainly showed G9(66.4%, n=156), G3(18.7%, n=44), G1(8.9%, n=21)and G8(1.7%, n=4).P typing mainly showed P[8](92.8%, n=218)and P[4](4.7%, n=11);G/P combination genotypes were composed mainly of G9P[8](57.0%, n=134), G3P[8](18.3%, n=43) and G1P[8](8.9%, n=21).The detection rate of RVA in diarrhea patients had obvious seasonal distribution (x2=46.3, P<0.001), with the prevalent peak in winter (31.2%), and the seasonal distribution of G9P[8](x2=27.3, P<0.001), G1P[8](x2=8.2, P<0.039)and G3P[8](x2=10.2, P<0.042) had strong seasonal pattern with a peak in winter equally.In the subjects under five years, the detection rate of G9P[8](14.9%, 2.9%, x2=18.1, P<0.001) and G3P[8](4.4%, 0.5%, x2=5.6, P<0.018) from diarrhea cases were higher than that of in healthy controls, respectively.Conclusion There existed rather high infection rate of RVA in acute diarrhea cases in Yunnan province, which can be divided into a variety of genotypes, G9P[8]was the dominant genotype.
More- 浏览:201
- 被引:5
- 下载:88
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文