摘要目的 检测锌指蛋白139(ZNF139)与基质金属蛋白酶(MMP)-2、MMP-7、基质金属蛋白酶组织抑制剂(TIMP)-1在胃癌原发灶、区域淋巴结转移灶中的表达,并探讨其意义.方法 应用逆转录-聚合酶链反应(RT-PCR)及免疫组织化学链菌素抗生物素蛋白-过氧化物酶(SP)法检测46例进展期胃癌患者的原发灶和淋巴结转移灶中ZNF139、MMP-2、MMP-7、TIMP-1 mRNA和蛋白水平.结果 胃癌区域淋巴结转移灶中ZNF139、MMP-2、MMP-7 mRNA表达分别为0.63±0.09、0.75±0.11、0.73±0.11,蛋白阳性率分别为80.43％、84.78％、86.96％,均明显高于原发灶的mRNA (0.32±0.05、0.27±0.04、0.21±0.03)和蛋白阳性率(54.35％、52.17％、58.70％)；而转移灶TIMP-1 mRNA(0.63±0.09)和蛋白阳性率(43.48％)则明显低于原发灶(1.24±0.18、78.26％,P＜0.05).ZNF139与MMP-2、ZNF139与MMP-7的在原发灶中mRNA表达均呈正相关(r分别为0.3258、0.3446,P＜0.05),ZNF139与TIMP-1无明显相关(P＞0.05)；ZNF139与MMP-7在淋巴结中mRNA表达水平呈正相关(r=0.3866,P＜0.05),而ZNF139与MMP-2、ZNF139与TIMP-1无明显相关(P＞0.05).结论 ZNF139可能通过调节部分侵袭相关基因的表达参与了胃癌的侵袭转移.

abstractsObjective To investigate the expressions and significance of zinc finger protein 139 (ZNF139),matrix metalloproteinase (MMP)-2,MMP-7,tissue inhibitor of metalloproteinase (TIMP)-1 in primary tumors (PT) and local lymph node metastases (LNMs) of gastric carcinoma.Methods The expression levels of ZNF139,MMP-2,MMP-7 and TIMP-1 were detected by using reverse transcriptasepolymerase chain reaction (RT-PCR) aod immunohistochemistry in 46 paired PT and LNMs of gastric cancer.Results The expression levels of ZNF139,MMP-2 and MMP-7 mRNA (0.63 ± 0.09,0.75 ±0.11,0.73 ± 0.11) and positive rate of proteins (80.43％,84.78％,86.96％) were all higher in LNMs than the mRNA expression levels (0.32 ±0.05,0.27 ±0.04,0.21 ±0.03) and positive rate of proteins (4.35％,52.17％,58.70％) in PT,whereas the expression of TIMP-1 mRNA and positive rate of protein were lower in LNMs (0.63 ± 0.09,43.48％) than in PTs (1.24 ± 0.18,78.26％) (all P ＜ 0.05).In PT there was a positive correlation hetween ZNF139 and MMP-2 or MMP-7 mRNA (r =0.3258,0.3446,both P ＜ 0.05).In LNMs,positive correlation was found between ZNF139 and MMP-7 (r =0.3866,P ＜0.05).Conclusion ZNF139 may be invovled in invasion and metastasis of gastric cancer by regulting some genes.