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目的 探讨血吸虫病门脉高压小鼠肠系膜静脉组织白细胞介素-1受体相关激酶-4(IRAK-4)的表达变化及意义.方法 将55只雄性昆明小鼠随机分为对照组(n=15)和感染组(n=40).感染组采用腹部敷贴法感染日本血吸虫尾蚴,分别于感染后12、20周随机分为12周组(n=20)、20周组(n=20)；采用逆转录-聚合酶链反应(RT-PCR)法检测肠系膜静脉IRAK-4 mRNA表达与肿瘤坏死因子(TNF)-α mRNA表达,Western blot法检测肠系膜静脉IRAK-4蛋白表达.结果 感染组小鼠肠系膜静脉IRAK-4 mRNA表达强度值(12周组0.734±0.081,20周组0.682±0.069)和IRAK-4蛋白表达水平(12周组:1034.52±89.72,20周组:989.56±69.35)较对照组(IRAK-4mRNA表达强度值0.163±0.031,IRAK-4蛋白表达水平667.45±72.22)明显升高(P＜0.01,P＜0.05);20周组IRAK-4 mRNA和蛋白表达与12周组比较差异无统计学意义(P＞0.05)；各组IRAK-4mRNA的表达与其效应分子TNF-α mRNA的表达呈显著正相关(r=5.56,P＜0.01).结论 门静脉高压症小鼠内脏血管IRAK-4在转录和翻译水平的表达升高,并激活下游炎性反应,是参与门静脉高压性血管病变的重要因素.

Objective To explore the expression and significance of interleukin-1 receptor associated kinase-4 (IRAK-4) in mesenteric vein of rats with schistosomal cirrhosis.Methods Fifty-five rats were randomly divided into two groups:normal control group (N group,n =15)) and model group (M group,n =40).The rats in M group were infected percutaneously with cercariae of schistosomiasis japonica,and divided into post-infection 12-week subgroup and 20-week subgroup.The mRNA expression levels of IRAK-4 and tumor necrosis factor (TNF)-α were detected by using reverse transcriptase polymerase chain reaction (RT-PCR).Western blotting was applied to examined the protein expression of IRAK-4.Results The expression of IRAK-4 mRNA in mesenteric vein in M groups (12th week:0.734 ± 0.081 ;20th week:0.682 ±0.069) was significantly stronger than in N group (0.163 ±0.031).The expression of IRAK-4 protein in M group (12th week:1034.52 ± 89.72 ; 20th week:989.56 ± 69.35) was significantly stronger than in N group (667.45 ± 72.22).No significant differences were found in the level of IRAK-4 between post-infection 20-week group and post-infection 12-week group (P ＞ 0.05).The expression of IRAK-4 mRNA was significantly positively correlated with the level of TNF-α mRNA (r =5.56,P ＜0.01).Conclusion The high expression of IRAK-4 and activation of downstream inflammatory factor play an important role in vasculopahty of rats with schistosomal cirrhosis.