大肠癌组织中肿瘤睾丸抗原前列腺相关基因4的表达及其临床意义
Cancer testis antigens prostate-associated gene 4 expression in colorectal cancer tissues and clinical implication
目的 筛选大肠癌相关肿瘤睾丸抗原,为大肠癌肿瘤的诊断和免疫治疗提供依据.方法 样本取自本院的大肠癌患者46例,包括癌组织(原发灶)、癌旁组织.提取样本核糖核酸(RNA)后逆转录合成单链互补脱氧核糖核酸(cDNA),利用聚合酶链反应(PCR)技术测定前列腺相关基因4 (PAGE4)在各组织中的相对表达量并进行分析.结果 有转移患者癌组织中PAGE4相对表达量(9.85×10-4 ±3.67×10-3)与无转移患者癌组织中相对表达量(3.93 ×10-5±1.20×10-4)差异有统计学意义(P<0.05);有转移患者癌组织中PAGE4表达阳性率(25%)与癌旁组织(0)比较差异有统计学意义(P<0.05);无转移患者癌组织中PAGE4表达阳性率(19.2%)与癌旁组织(11.5%)比较差异无统计学意义(P>0.05).结论 PAGE4的表达可作为肿瘤转移的预测因素,转移性大肠癌者可能是针对PAGE4标靶的肿瘤免疫治疗的适宜人群.
更多Objective To identify the cancer testis antigens associated with colorectal cancer for clinical diagnosis and immunotherapy.Methods The expression level of prostate-associated gene 4 (PAGE4) was detected by polymerase chain reaction (PCR) in the specimens,including cancer tissues,and cancer adjacent tissues from colorectal cancer cases (n =46) in our hospital.Results There was significant difference in the PAGE4 expression between metastatic tissues (9.85 × 10-4 ±3.67 × 10-3) and non-metastatic cancer tissues (3.93 × 10-5 ± 1.20 × 10-4) (p < 0.05).There was significant difference in PAGE4 positive expression rate between cancer tissues (25%) and cancer adjacent tissues (0) in metastatic cancer cases (P < 0.05).There was no significant difference in PAGE4 positive expression rate between cancer tissues (19.2%) and cancer adjacent tissues (11.5%) in non-metastatic cancer cases (P > 0.05).Conclusion PAGE4 might be potential predictors for metastasis of colorectal cancer and cancer testis antigen-targeted immunotherapy might be indicated for those cases with metastasis.
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