摘要目的 观察鞘内注射程序性坏死特异性抑制剂-1(Nec-1)对大鼠对脊髓损伤修复的促进作用.方法 SD大鼠30只,采用改良Allen's法建立大鼠脊髓损伤模型,按照随机数字表法分为3组,假手术组、生理盐水组和Nec-1治疗组,每组10只.脊髓损伤后7d对各组大鼠进行运动功能评价、运动诱发电位测定、脊髓坏死测定.结果 术后7、14、21 d Nec-1治疗组大鼠运动功能恢复均好于生理盐水组,运动功能评分显著高于生理盐水组,差异均有统计学意义(P＜0.05).术后7d时,Nec-1治疗组大鼠皮层运动诱发电位(MEP)潜伏期变化为(71.37 ±12.71)％,显著低于生理盐水组[(104.5±14.57)％,t=8.147,P＜0.01],波幅峰值变化为(69.27±4.75)％,也显著小于生理盐水组[(87.93±5.47)％,t=3.201,P＜0.05].术后7d时,Nec-1治疗组脊髓损伤坏死区为(6.91±0.42) mm2,较生理盐水组脊髓损伤坏死区范围显著减小[(11.29 ±0.63) mm2,t=7.542,P＜0.01).术后7d时,Nec-1治疗组存活神经元细胞计数为(10.54±1.27)个,较生理盐水组显著增多,差异有统计学意义[(5.27±0.47)个,t=6.341,P＜0.01];染色髓鞘吸光度(A)相对值Nec-1治疗组为0.94±0.06,较生理盐水组显著增多,差异有统计学意义(0.67±0.08,t=5.574,P＜0.01).结论 Nec-1可以改善大鼠运动功能及皮质运动诱发电位的恢复,对脊髓损伤修复具有促进作用.

abstractsObjective To investigate the protective role of Necrostain-1 (Nec-1) in spinal cord injury in rats.Methods Rat spinal cord injury models were established according to the Allen's method with some modifications,and randomly divided into three groups:sham operated group,saline group and Nec-1 treatment group (n =10 per group).The behavioral function,evoked potential and necrosis of spinal cord were measured at 7th day after injury.Results At 7th,14th and 21st day after injury,the Basso-Beattie-Bresnahan (BBB) scores in Nec-1 treatment group were significantly higher than in saline group (P ＜ 0.05),and similar to those in the sham operated group (P ＞ 0.05).At 7th day after injury,the latency of MEP in Nec-1 treatment group was (71.37 ± 12.71) ％,significantly lower than that of saline group [(104.5 ± 14.57)％,t =8.147,P ＜ 0.01].In addition,the latency in Nec-1 treatment group was (69.27 ± 4.75) ％,significantly lower than that in saline group [(87.93 ± 5.47) ％,t =3.201,P ＜ 0.05].Moreover,the necrotic area of injured spinal cord in Nec-1 treatment group was (6.91 ± 0.42) mm2,significantly lower than that in saline group [(11.29 ± 0.63) mm2,t =7.542,P ＜ 0.01].The survival number of neurons in Nec-1 treatment group was 10.54 ± 1.27,significantly more than that in saline group (5.27 ±0.47,t =6.341,P ＜0.01).The relative A of myelin in Nec-1 treatment group was (0.94 ± 0.06),significantly higher than that in saline group (0.67 ± 0.08,t =5.574,P ＜ 0.01).Conclusion Nec-1 has a neuroprotective effect on spinal cord injury through promoting motor function and MEP recovery in rats.