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白细胞介素-1β和基质金属蛋白酶-9在兔膝关节骨性关节炎模型滑膜中的表达及意义

Expression and significance of interleukin-1 beta and matrix metalloprotein-9 in synovial membrane of rabbit knee osteoarthritis model

摘要:

目的 通过建立兔膝关节骨性关节炎(OA)模型,探讨白细胞介素(IL)-1β、基质金属蛋白酶(MMP).9在OA滑膜中的表达及其意义.方法 60只新西兰大白兔随机分为6组,A1、A2、A3组为模型组,B1、B2、B3组为对照组.A1、A2、A3组用1.6%木瓜蛋白酶膝关节腔内注射,建立兔膝关节OA模型,分别在2、4、6周后处死,观察大体标本形态学变化及标本滑膜中IL-1β和MMP-9的表达情况.结果 (1)实验组4周和8周软骨细胞IL-1β和MMP-9细胞分数明显高于对照组,差异有统计学意义(P<0.05或P<0.01),实验组软骨细胞IL-1β和MMP-9细胞分数4周较8周组明显增高,差异有统计学意义(P<0.05).(2)酶联免疫吸附试验(ELISA)法检测IL-1β、MMP-9的表达:A1组>B1组、A2组>B2组、A3组>B3组(P<0.05),模型组中A1组<A2组、A2组<A3组(P<0.05).(3) Western blot法检测IL-1β蛋白的相对表达水平:模型组滑膜中的IL-1β3的相对表达量均高于对照组,A1组(0.80±0.05) >B1组(0.40±0.02)、A2组(1.08±0.06) >B2组(0.51±0.03)、A3组(1.26±0.08)>B3组(0.61 ±0.03,P<0.01),且随实验进展时间的延长而增加.A1组< A2组,A2组<A3组(P<0.05).结论 IL-1β和MMP-9的差异表达与OA退变的发展密切相关.

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Objective To investigate the expression and significance of matrix metalloprotein-9 (MMP-9) and interleukin (IL)-1β in the synovial membrane of osteoarthritis (OA) model.Methods Sixty New Zealand white rabbits were randomly divided into 6 groups:A1,A2,A3 groups as model groups,and B1,B2,B3 groups as the control groups.In A1,A2 and A3 groups,1.6% papain was injected into the articular cavity to establish the OA model of the knee joints,and the animals were sacrificed at 2nd,4th and 6th week.The expression of MMP-9 and IL-1β in the specimens was observed.Results (1) At 4th week and 8th week,the MMP-9 and IL-1β expression levels in the model groups were significantly higher than those in the control groups (P < 0.05 and P < 0.01).At 4th week,the MMP-9 and IL-1β levels in the model groups were significantly higher than those at 8th week (P < 0.05).(2)The expression levels of IL-1β and MMP-9 in group A1 were higher than in group B1,group A2 higher than in group B2,group A3 higher than in group B3 (P < 0.05),group A1 lower than in group A2 and group A2 lower than in group A3 (P < 0.05).(3) For Western blotting,the content of IL-1β in model groups was higher than in control groups:group A1 (0.80 ± 0.05) higher than in group B1 (0.02 ±O.40),group A2 (1.08 ± 0.06) higher than in group B2 (0.06 ± 1.08),group A3 (0.03 ± 0.08) higher than in group B3 (0.61 ±0.03,P <0.01),and increased with the time in the model groups:group A1 lower than in group A2,and group A2 lower than in group A3 (P < 0.05).Conclusion The differential expression of IL-1β and MMP-9 is closely related to the development of OA degeneration.

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