依托咪酯对过氧化氢诱导的PC12细胞损伤的影响及机制
Mechanism and effect of etomidate on H2O2 induced PC12 cell injury
目的 探讨依托咪酯对过氧化氢(H2O2)诱导的PC12细胞损伤的保护作用.方法 PC12细胞随机分为正常组,H2O2组(200 μmol/L H2O2),依托咪酯低、中、高浓度组,并分别检测各组细胞活力、凋亡、半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3、Caspase-9活性、丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性,B淋巴细胞瘤-2(bcl-2)及bcl-2相关X蛋白(bax)蛋白表达.结果 与正常组比较,H2O2组(0.38±0.03比0.65±0.06)细胞活力降低(P<0.01),细胞早期[(15.64±1.56)%比(2.32±0.23)%]及晚期凋亡率[(24.61±2.46)%比(2.09±0.21)%](P<0.01)、MDA含量(P<0.01)、Caspase-3[(4.39±0.44)比(0.58±0.05)](P<0.01)、Caspase-9[(2.95±0.29)比(0.48±0.04)]活性提高(P<0.01),SOD、CAT及GSH-Px活性降低(P<0.01),bcl-2表达量下调(P<0.01),bax表达量上调(P<0.01).与H2O2组比较,依托咪酯低、中、高浓度组细胞活力提高(P<0.01),细胞凋亡率(P<0.01)、MDA含量(P<0.01)、Caspase-3、Caspase-9活性降低(P<0.01;P<0.01),SOD(P<0.01)、CAT(P <0.01)及GSH-Px(P<0.01)活性提高,bcl-2表达量上调(P<0.01),bax表达量下调(P<0.01).结论 依托咪酯可能通过提高抗氧化能力及抵抗细胞凋亡抑制H2O2引起的PC12细胞损伤.
更多Objective To explore protective effect of etomidate on H2O2 induced PC12 cell injury.Methods PC12 cell was randomized into normal group,H2O2 group (200 μmol/L H2O2),etomidate low,medium and high-dose group.Cell viability,cell apoptotic rate,malondialdehyde (MDA) content,the activity of eysteinyl aspartate specific proteinase (Caspase)-3,Caspase-9,superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px),the expression of B-cell lymphoma-2 (bcl-2) and bcl-2 associated X protein (bax) was measured.Results Compared with normal group,cell viability [(0.38±0.03) vs.(0.65 ± 0.06)] was decreased (P < 0.01),the cell late apoptotic rate [(15.64 ±1.56)% vs.(2.32 ±0.23)%],cell late apoptotic rate [(24.61 ±2.46)% vs.(2.09 ±0.21)%],the activity of Caspase-3 [(4.39 ±0.44) vs.(0.58 ±0.05)],Caspase-9 [(2.95 ±0.29) vs.(0.48 ±0.04)],MDA content was increased (P < 0.01),the activity of SOD,CAT and GSH-Px was decreased (P <0.01),the expression of bcl-2 was down-regulated (P < 0.01),the expression of bax was up-regulated (P < 0.01).Compared with H2O2 group,cell viability was increased (P < 0.01),the cell apoptosis rate (P < 0.01),the activity of Caspase-3 (P < 0.01),Caspase-9 (P < 0.01),MDA content was reduced (P<0.01),the activity of SOD (P<0.01),CAT (P<0.01) and GSH-Px (P<0.01) was increased,the expression of bc1-2 was up-regulated (P <0.01),the expression of bax was down-regulated (P <0.01).Conclusion Etomidate could suppress H2O2 induced PC12 cell injury via increasing antioxidant capacity and resistance to cell apoptosis.
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