摘要目的探讨外源性hBMP2基因在成纤维细胞获得稳定表达的可行性。方法将hBMP2的cDNA连入真核表达载体pcDNA3，形成重组真核表达载体pcDNA3-hBMP2，在脂质体介导下，将其导入小鼠成纤维细胞株(NIH3T3)。通过G418筛选获得阳性克隆，并继续培养4周，然后用原位杂交和免疫组织化学方法检测BMP2基因在成纤维细胞NIH3T3内的稳定表达情况。结果经原位杂交和免疫组织化学证实，转染pcDNA3-hBMP2后的成纤维细胞NIH3T3内有大量hBMP2 mRNA的转录和蛋白的表达。结论在脂质体介导下，hBMP2基因能够导入成纤维细胞内并在其内获得稳定表达。

abstractsObjective　To examine the effectiveness of a gene transfer of human bone morphogenetic protein (BMP)-2 into fibroblasts NIH3T3.　Methods　pcDNA3-hBMP2　was constructed using gene clone and recombined technique. With the help of lipofectamine, fibroblasts NIH3T3 was transfected with pcDNA3-hBMP2. The positive cell clones were selected with G418.The stable transfection and expression of hBMP2 in the fibroblasts NIH3T3 were determined by in situ hybridization and immunohistochemical analysis.　Results　The two fragments digested from pcDNA3-hBMP2 by EcoRI and XbaI represented 5.38 kb and 1.3 kb　by electrophoresis, which were confirmed to be the carrier and the hBMP2 gene fragments inserted originally, indicating that the construction of pcDNA3-hBMP2 was successful. Abundant BMP2 stable expression in fibroblasts NIH3T3 transfected with pcDNA3-hBMP2 was confirmed by in situ hybridization and immunohistochemical analysis.　Conclusion　With the help of lipofectamine, hBMP2 gene can be transferred and stably expressed in fibroblasts NIH3T3.