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血管生成因子VEGF、bFGF、endostatin在胰腺癌细胞中的表达

Expression of angiogenic factors in pancreatic carcinoma cell and their significance

摘要:

目的 探讨胰腺癌细胞血管生成因子表达水平与其恶性生物学行为的关系.方法 通过RT-PCR及ELISA检测胰腺癌细胞株SW1990、Capan-1、Aspc-1、MiaPaCa-2、Panc-1及PCT-3中血管内皮生长因子(VEGF)、碱性成纤维生长因子(bFGF)和内皮素(endostatin)的表达水平,同时通过Boyden Chamber趋化小室及CCK-8法进行6株胰腺癌细胞株的侵袭性及增殖检测. 结果 VEGF在6株细胞中均有表达,但是表达量存在显著差异,以侵袭性最强的Panc-1表达最强.bFGF在6株细胞中均有表达,但是表达量存在显著差异,增殖及侵袭性较强的Panc-1和PCT-3中的表达水平高于其他细胞株.6株胰腺癌细胞endostatin表达差异显著,在侵袭性最强的Panc-1中表达水平最高,其他细胞株不表达或表达量很低.胰腺癌细胞株胞外的VEGF及endostatin表达显著高于细胞内,bFGF细胞内表达显著高于细胞外.结论 血管生成因子VEGF、bFGF和endostatin的表达水平可能与胰腺癌细胞增殖与侵袭密切相关.

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abstracts:

Objective To investigate the relationship between expression of angiogenic factors and invasion and proliferation in pancreatic carcinoma cell. Methods Expressions and secretions of angiogenic factors in pancreatic carcinoma cell lines were determined by RT-PCR and ELISA. Proliferation and invasion of pancreatic carcinoma cell lines were determined by CCK-8 and Boyden Chamber invasion tests.ResultsPancreatic carcinoma cell lines showed significantly different ability of invasion and proliferation. Intro-cellular VEGF expressions of SW1990,Capan-1,Aspc-1,MiaPaCa-2,Panc-1 and PCT-3 were (152.9±0.6),(224.1±60.3),(239.2±2.1),(19.3±0.7),(165.6±34.3),and (18.1±1.4)pg/(106cell*24 h). Extro-cellular VEGF expressions of SW1990,Capan-1,Aspc-1,MiaPaCa-2,Panc-1 and PCT-3 were (1331.1±67.8),(3902.6±79.7),(2657.3±51.9),(1498.3±4.8),(4696.8±45.5),and (1200.5±42.2)pg/(106cell*24 h). Intro-cellular bFGF expressions of SW1990,Capan-1,Aspc-1,MiaPaCa-2,Panc-1 and PCT-3 were (66.1±4.8),(206.8±99.5),(1532.0±54.6),(159.2±11.0),(1612.0±515.9) and (2781.2±479.0)pg/(106cell*24 h). Extro-cellular bFGF expressions of SW1990,Capan-1,Aspc-1,MiaPaCa-2,Panc-1 and PCT-3 were (2.1±0.6),(10.3±1.5),(31.0±0.4),(4.3±1.2),(43.6±1.5) and (82.1±10.4)pg/(106cell*24 h). Intro-cellular endostatin expressions of SW1990,Capan-1,Aspc-1,MiaPaCa-2,Panc-1 and PCT-3 were (0.2±0.0),(0.3±0.0),(4.7±0.1),(10.8±0.2),(31.9±11.7) and (5.4±0.1)ng/(106cell*24 h). Extro-cellular endostatin expressions of SW1990,Capan-1,Aspc-1,MiaPaCa-2,Panc-1 and PCT-3 were (0.0±0.0),(1.6±0.0),(21.5±1.1),(40.8±0.4),(129.2±1.0) and (20.1±1.8)ng/(106cell*24 h). Panc-1 enjoying stronger invasion and proliferation showed stronger expressions of VEGF,bFGF and endostatin. Intro-cellular expressions of bFGF was stronger than extro-cellular,extro-cellular expressions of VEGF and endostatin were stronger than intro-cellular. Conclusion Expressions of angiogenic factors regulated by cancer cell played an important role in progression of pancreatic carcinoma.

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