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芦荟多糖预处理对初进高原重度失血性休克大鼠脑组织炎性和脂质过氧化反应的影响

Effect of aloe polysaccharides pretreatment on the cerebral inflammatory response and lipid peroxidation in severe hemorrhagic shock rats first entering high altitude

摘要:

目的 探讨芦荟多糖预处理对初进高原重度失血性休克大鼠脑组织炎性和脂质过氧化反应的影响.方法 雄性SD大鼠40只,体质量250~ 300 g.随机分为5组(n=8):假手术组、休克组和芦荟多糖预先给药(AP)组,AP组按剂量分为0.75 mg/kg(AP1)、1.50 mg/kg( AP2)和3.00 mg/kg(AP3)3个亚组.假手术组仅行动静脉穿刺,不放血,休克组仅制备重度失血性休克模型,AP1、AP2、AP3组分别于放血前30 min股静脉注射不同剂量AP,随后制备重度失血性休克模型.复苏后2h从右股动脉抽血3ml,随后立即处死大鼠,冰上取脑组织,分离海马及额顶叶脑组织.测定血清肿瘤坏死因子α(TNF-α)、IL-6及IL-10水平和海马组织中丙二醛含量和髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性及脑湿干质量比.结果 与假手术组比较,休克组血浆TNF-α[(76±11)ng/L]、IL-6[ (1303±141) ng/L]及IL-10含量[(95±14) ng/L]、海马组织丙二醛含量[(80±13)nmol/mgprot]、MPO活性[(20.72±2.28)×10-2 U/g]及脑湿干质量比(6.21±0.18)升高(t=6.928 ~14.565,P<0.05),而SOD活性[(56±11) U/mgprot]降低(t=-5.374,P<0.05);休克组和AP1组间上述各指标差异均无统计学意义;与休克组比较,AP2组血浆TNF-α[( 54±12) ng/L]、IL-6[(846±78) ng/L]及IL-10含量[(66±11)ng/L]、海马组织丙二醛含量[(56±9) nmol/mgprot]、MPO活性[( 13.13±1.23)× 10-2U/g]及脑湿干质量比(5.71±0.18)降低(t=-6.905~ -3.357,P<0.05),而SOD活性[(86±12) U/mgprot]升高(t=4.240,P<0.05);AP2和AP3组间上述各指标差异均无统计学意义.结论 芦荟多糖预处理可抑制失血性休克大鼠脑组织炎性反应和氧自由基反应.

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abstracts:

Objective To investigate the effect of aloe polysaccharides pretreatment on the cerebral inflammatory response and lipid peroxidation in severe hemorrhagic shock rats first entering high altitude.Methods Forty healthy male SD rats weighing 250-300 g were randomly divided into 5 groups ( n =8 each):sham group,shock group,AP group was further divided into 3 subgroups (AP1 0.75 mg/kg; AP2 1.50 mg/kg; AP3 3.00 mg/kg).The different doses AP were given iv respectively at 30 min before hemorrhagic shock.The mean blood pressure (MAP) was maintained at (35 ±5) mmHg (1 mmHg =0.133kPa) for 60 minutes.The animals were killed at 2 hours after resuscitation.Blood samples were obtained from femoral artery for detecting tumor necrosis factor α (TNF-α),IL-6 and IL-10 concentrations; the frontal and parietal lobes brain and the hippocampus were separated from brain tissues on the ice for detecting superoxide dismutase (SOD) activity and myeloperoxidase (MPO) activity,malondialdehyde (MDA) concentration,brain Wet-dry weight ratio (W/D).Results Compared with sham group,hemorrhagic shock significantly increased serum TNF-α ( (76 ± 11 ) ng/L),IL-6 ( ( 1303 ± 141 ) ng/L)and IL-10 concentrations ( ( 95 ± 14 ) ng/L),MPO activity ( ( 20.72 ± 2.28 ) × 10-2 U/g) and MDA concentration ( (80 ± 13 ) nmol/mgprot) in the brain tissue and brain W/D (6.21 ± 0.18) ( t =6.928-14.565,P < 0.05 ),while SOD activity ( (56 ±11 ) U/mgprot) decreased significantly ( t =- 5.374,P <0.05 ).There were no significant difference between shock and AP1 groups.AP2 group significantly inhibited hemorrhagic shock-induced increase serum TNF-α ( (54 ± 12) ng/L),IL-6 ( (846 ±78) ng/L)and IL-10 concentrations ( ( 66 ± 11 ) ng/L),MPO activity ( ( 13.13 ± 1.23 ) × 10-2 U/g) and MDA concentration ((56±9) nmol/mgprot) in the brain tissue and brain W/D (5.71±0.18) (t=-6.905--3.357,P <0.05),while SOD activity ( ( 86 ± 12) U/mgprot) increased significantly compared to shock group ( t =4.240,P < 0.05 ).There were no significant difference between AP2 and AP3 groups.Conclusion AP pretreatment can attenuate the cerebral ischemia and reperfusion injury in severe traumatic-hemorrhagic rats first entering high altitude through inhibiting systemic inflammatory response and leukocyte aggregation and lipid peroxidafion in the brain.

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作者: 路建 [1] 肖旺频 [1] 耿智隆 [2] 刘东 [2] 王英峰 [2]
第一作者: 路建
作者单位: 嘉兴学院附属第二医院麻醉科 [1] 730050,兰州军区兰州总医院麻醉科 [2]
期刊: 《中华外科杂志》2012年50卷7期 655-658页 MEDLINEISTICPKUCSCD
分类号: R605.971
栏目名称: 论著
DOI: 10.3760/cma.j.issn.0529-5815.2012.07.021
发布时间: 2012-10-29
基金项目:
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