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运动训练对脑出血大鼠LINGO-1表达的影响

Effects of exercise on the expression of the LINGO-1 gene in rats after intra-cerebral hemorrhage

摘要:

目的 探讨运动训练对脑出血大鼠神经功能恢复和脑内LINGO-1表达的影响.方法 选取72只SD大鼠按随机数字表法分为假手术组、模型组和运动训练组,每组24只,各组再按观察时间的不同随机分为1、3、7和14d四个时间点,每个时间点6只大鼠.制备脑出血模型,运动训练组采用跑台跑步法进行跑步运动训练.各组大鼠采用Longa评分法评价神经功能缺损程度,RT-PCR法检测LINGO-1 mRNA的表达,Western blot法检测LINGO-1蛋白的表达.结果 假手术组神经功能损伤轻,Longa评分0或1分,各时间点LINGO-1 mRNA和蛋白表达均仅少量表达.模型组Longa评分7d最高达3分,14d出现下降到2分;LONG-1mRNA 3 d表达最高达0.737±0.041,7d出现下降为0.429±0.035;LONG-1蛋白7d到高峰达1.335±0.393,14d出现下降为1.225±0.424.运动训练组在14 d Longa评分为1分,较模型组下降(P<0.05);在7d和14dLINGO-1 mRNA表达分别为0.257±0.042和0.137±0.035,较模型组下降(P<0.05);在14d LINGO-1蛋白表达为0.663±0.276,较模型组下降(P<0.05);且各时间点LINGO-1蛋白的表达与Longa评分成正相关(r1d=0.837,P1d=0.036;r3d=0.845,P3d=0.047;r7d=0.891,P7d=0.029;r14d=0.926,P14d=0.018).结论 运动训练可下调LINGO-1 mRNA和蛋白的表达,促进脑出血后神经功能恢复.

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Objective To explore the effect of exercise on the expression of the LINGO-1 gene after intra cerebral hemorrhage (ICH).Methods Seventy-two Sprague-Dawley rats were randomly divided into a sham operation group,a control group and an exercise training group,each of 24.The three groups were further divided into 1 d,3 d,7 d and 14 d groups.ICH was induced using intra-parenchymal injection of autologous blood.The exercise training group was then forced to exercise on a treadmill.Any recovery of neurological functional was evaluated using Longa scoring,and the expression of LINGO-1 mRNA and protein were detected using the reverse transcription-polymerase chain reaction and western blotting.Results The sham operation group displayed no obvious neurological deficiency,with little expression of LINGO-1 mRNA or protein at any time point.The control group's average Longa score reached a maximum of 3 seven days after the operation,decreasing to 2 after another 7 days.The average expression of LINGO-1 mRNA and protein in that group peaked at 1.335±0.393 three days after the ICH,then decreased to 0.429±0.035 on the 7th day and 0.371±0.038 on the 14th day.In the exercise training group the average Longa score on the 7th day was 2,the average LINGO-1 mRNA level was 0.257±0.042 and the average protein level was 1.142±0.287,all significantly lower than in the control group.Moreover,in the exercise group there was a significant positive correlation between LINGO-1 protein expression and the Longa scores.Conclusion Exercise can decrease the expression of LINGO-1 mRNA and protein and promote the recovery of neural function after ICH.

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作者: 梁顺利 [1] 吴忧 [1] 张荣博 [1] 章水晶 [1] 徐彬 [1]
栏目名称: 基础研究
DOI: 10.3760/cma.j.issn.0254-1424.2017.09.001
发布时间: 2017-11-22
基金项目:
浙江省医药卫生科技计划项目(2014KYB179)Zhejiang Province Medical and Health Science and Technology Project
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