CD147在亲环素A诱导Jurkat细胞增殖、活化和趋化过程中的作用
The role of CD147 in the proliferation,activation and chemotaxis of Jurkat cell induced by cyclophilin A
目的 探讨CD147分子在亲环素A(CyPA)诱导的Jurkat细胞增殖、活化和趋化过程中的作用.方法 采用RT-PCR法和Western blot法证实CD147小干扰RNA(siRNA)转染Jurkat细胞的有效性.不同浓度CyPA(0.01、0.10、1.00、10.00 nmol/L)及PBS作用于CD147 siRNA转染前后的Jurkat细胞,采用MTT法检测CyPA作用24和48 h后细胞增殖水平;流式细胞术检测细胞表面T细胞活化标志分子CD25的表达;以Transwell小室法和基质黏附实验分别检测细胞趋化能力和黏附能力.结果 CD147 siRNA转染的Jurkat细胞CD147mRNA和蛋白表达水平较空转细胞显著下降;CyPA以浓度依赖性方式促进Jurkat细胞增殖,CyPA浓度为10.00 nmol/L时促增殖作用最强,阻断CD147表达使CyPA促细胞增殖作用下降;CyPA可促进Jurkat细胞的活化,阻断CD147表达致CyPA促细胞活化能力下降;CyPA对Jurkat细胞有趋化作用,趋化指数为2.32,阻断CD147表达后细胞趋化活性显著降低;CyPA对Jurkat细胞的黏附能力无明显影响.结论 CD147分子在CyPA诱导的Jurkat细胞活化、增殖和趋化过程中发挥作用.
更多Objective To investigate the role of CD147 in the proliferation,activation and chemotax is of Jurkat cell induced by cyelophilin A(CyPA).Methods CD147 mRNA and protein level siRNA trans fected Jurkat ceils were identified by RT-PCR and Western blot respectively.Jurkat cell,Jurkat-vector cell and Jurkat-CD147siRNA cells were treated with different concentrations of CyPA (0.01 nmol/L,0.1 nmol/L,1 nmol/L,10 nmol/L) or PBS for 24 h and 48 h.Proliferation level was detected by MTF assay.CD25 was measured by flow cytometry.Transwell chamber was used to detect the chemotaxis.The effect of CyPA on the adhesive potential of Jurkat cell was studied by cell-matrix adhesion assay.Results CD147 mRNA and protein level siRNA transfected cells were decreased significantly than that of control cells.CyPA stimulated the proliferation of Jurkat cell in a dose-dependent manner,its effect peaked at 10 nmol/L CyPA.Blockage of CD147 expression decreased the proliferation level of Jurkat cell induced by CyPA.CyPA increased the activation rate of Jurkat cell,and blockage CD147 expression decresed the activation rate of the cell induced by CypA.CyPA showed a chemotatic activity on Jurkat cell,the chemotaxis index being 2.32,and the chemotactic ability was decreased after inhibition of CD147 expression.CyPA had no effect on adhesion of Jurkat cell to extraceilular matrix.Conclusion CD147 plays a role in the proliferation,activation and chemotaxis of Jurkat cell induced by CyPA.
More- 浏览:250
- 被引:10
- 下载:40
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文