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胎盘组织血凝素样氧化低密度脂蛋白受体-1与早发型子痫前期的关系

Expression of lectin-liked oxidized low density Hpoprotein receptor-1 and apoptosis associated genes in placenta and the relationship thereof with morbUity of early-onset preeelampsia

摘要:

目的 探讨血凝素样氧化低密度脂蛋白受体-1(LOX-1)与早发型子痫前期发病的关系.方法 比较60名正常孕妇和同孕周30例早发型及30例晚发型子痫前期患者胎盘中LOX-1和凋亡相关基因表达的时空变化;以免疫组化、RT-PCR和Western印迹方法检测同孕周正常孕妇和早发型及晚发型子痫前期患者胎盘组织中LOX-1和凋亡相关基因Caspase-3、Bax及Bcl-2的表达;以妊娠32周作为早发型子痫前期的界定标准.结果 早发型子痫前期20周+1~24周、24周+1~28周、28周+1~32周孕妇胎盘组织中LOX-1蛋白表达水平灰度值分别为27.6±1.3、29.4±1.4、32.3±1.7,正常妊娠20周+1~24周、24周+1~28周、28周+1~32周LOX-1表达水平分别为11.2±0.6、18.7±1.0、25.5 4±1.6,两组比较均P<0.05;晚发型子痫前期32周+1~36周、36周+1~40周孕妇胎盘组织中LOX-1蛋白表达水平分别为29.3 ±1.5、32.3±1.3,正常妊娠32周+1~36周、36周+1~40周LOX-1表达水平分别为28.1±1.8、31.3±1.6,两组比较均P>0.05.早发型子痫前期20周+1~24周、24周+1~28周、28周+1~32周孕妇胎盘组织中Caspase-3蛋白表达水平分别为17.4±0.9、23.2±1.1、28.4±0.8,正常妊娠20周+1~24周、24周+1~28周、28周+1~32周Caspase-3表达水平分别为8.5 ±1.0、11.3±1.1、14.4±1.2,两组比较均P<0.05;晚发型子痫前期32周+1~36周、36周+1~40周孕妇胎盘组织中Caspase-3蛋白表达水平分别为29.3±0.5、33.3±1.1,正常妊娠32周+1~36周、36周+1~40周Caspase-3表达水平分别为22.4 ±1.5、27.2±1.3,两组比较均P<0.05.结论 oxLDL受体LOX-1可能与早发型子痫前期的发病有关.

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abstracts:

Objective To investigate the expression of lectin-liked oxidized low density lipoproteinreceptor-1 (LOX-1) and apeptosis associated genes: caspas-3, Bax, and Bcl-2, in placenta and therelationship thereof with the morbility of early-onset preeelampsia. Methods 30 placentas of normalpregnant women at the 21 + 1 to 32 weeks gestation, 30 patients with early-onset preeclampsia at the 21 + 1to 32 weeks gestation, 30 normal pregnant women at the 32 + weeks gestation to full term, and 30 patientswith late-onset proclampsia at the 32 + weeks gestation to full term. Immunohistochemistry, RT-PCR, andWestern-blotting were used to detect the expression of LOX-1, caspase-3, Bax, and Bcl-2 in the placenta.Results The LOX-1 protein expression levels (grey levels) in the placenta tissues at 20W+1 -24W,24W+1 -28W, and 28W+1 -32W gestation of the early-onset preeclampsia were 27.6 ± 1.3, 29.4 ± 1.4,and 32.3 ± 1.6 respectively, all significantly higher than those of the normal pregnant women (11.2 ± 0.6,18.7±1.0, and 25.5 ± 1.6 respectively, all P <0.05). The LOX-1 protein levels at the 32W+1 -36Wand 36W+1 -40W gestation of the late-onset preeclampsia patients were : 29.3 ± 1.5 and 32.3 ± 1.3respectively, not significantly different from those of the normal pregnant women (28.1±1.8 and 31.3 ±1.6 respectively, both P >0.05). The caspase-3 protein level Caspase-3 protein at 20W+1 -24W, 24W+1-28W, and 28W+1 -32W gestation of the early-onset preeclampsia patients were 17.4±0.9, 23.2±1.1,and 28.4 ± 0.8 respectively, all significantly higher than those of the normal pregnant women (8.5 ± 1.0,11.3 ± 1.1, and 14.4 ± 1.2 respectively, all P <0.05). The caspase-3 protein levels at 32W+1 -36W and36W+1- 40W gestation of the late-onset preeclampsia patients were 29.3 ± 0.5 and 33.3 ± 1.1respectively, all significantly higher than those of the normal pregnant women (22.4 ± 1.5 and 27.2 ± 1.3respectively, both P < 0.05). The Bax mRNA expression and protein expression of the early-onsetpreeclampsia patients were higher than those of the normal pregnant women, and the Bcl-2 mRNA expressionand protein expression of the early-onset preeclampsia patients were lower than those of the normal pregnantwomen. The Bax mRNA expression and protein expression of the late-onset preeclampsia patients were stillhigher than those of the normal pregnant women; however, the Bcl-2 mRNA expression and proteinexpression of the late-onset preeclampsia patients were not significantly different from those of the normalpregnant women. Conclusion LOX-1 may be concerned with the pathogenesis of early onset ofpreeclampsia.

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作者: 孟涛 [1] 陈海英 [2] 李俊 [3] 尚涛 [3]
期刊: 《中华医学杂志》2008年88卷37期 2633-2635页 MEDLINEISTICPKUCSCD
分类号: R71
栏目名称: 临床研究
DOI: 10.3321/j.issn:0376-2491.2008.37.009
发布时间: 2009-01-06
基金项目:
辽宁省自然科学基金
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