人类白细胞抗原-DRB基因与儿童哮喘的相关性
Association study between EILA-DRB genes and asthmatic children
目的 调查人类白细胞抗原(HLA)-DRB等位基因在哮喘患儿中的分布频率及其与哮喘严重程度的相关性.方法 采用序列特异性引物聚合酶链反应(PCR-SSP)对北京市117例哮喘患儿和120名健康儿童进行HLA-DRB基因分析,计算HLA-DRB各等位基因在哮喘患儿和正常儿童的分布频率、比值比(OR)值,筛查出与哮喘相关的易感基因或抗性基因.结果 HLA-DR2(15)等位基因在哮喘息儿组的频率显著高于对照组[12.0%(28/234)比5.4%(13/240),P=0.011,OR=2.590];而DR4、DR6(1402)、DR9、DR53等位基因在哮喘患儿组的频率[9.4%(22/234)、0.9%(2/234)、17.1%(40/234)、29.5%(69/234)]均显著低于对照组[16.3%(39/240)、5.0%(12/240)、31.3%(75/240)、44.2%(106/240),P=0.026、0.008、0.000、0.001,OR=0.481、0.157、0.312、0.190)];其他等位基因分布差异均无统计学意义.以DR2(15)、DR4、DR6(1402)、DR9、DR53及性别、家族史为自变量,进行哮喘发病相关因素的Logistic回归分析,各等位基因OR值的95%CI分别为1.010~2.245,0.757~1.116,0.603~1.054,0.855~1.014,0.971~1.010.对HLA-DR2(15)等位基因阳性和阴性的患儿与哮喘严重程度进行相关分析,差异无统计学意义.结论 HLA-DR2(15)为儿童哮喘的易感基因,但其与哮喘严重程度无关.
更多Objective To investigate the distribution of HLA-DRB alleles among the asthmatic children and find the alleles with a correlation with susceptibility or resistance to childhood asthma. Methods The sequence-specific polymerase chain reaction (SSP-PCR) was used to analyze the HLA-DRB genotype in 117 asthmatic children and 120 healthy children in Beijing. Then the frequency of each type, odds ratio (OR) and the 95% confidence interval (CI) of OR were calculated. Results The frequency of DR2. (15) in the asthmatic children was 12. 0% (28/234)vs 5.4% (13/240)in the healthy children. The frequency was significantly higher than that of nonasthmatic children ( OR = 2.590, 95% CI = 1. 266 - 5.298, X2 = 6.431, P <0.05). Conversely, the frequencies of DR4, DR6(1402), DR9 and DR53 in asthmatic children [9.4% (22/234),0.9% (2/234),17. 1% (40/234),29.5% (69/234) ]were significantly lower than that of the healthy children [ 16.3% (39/240) ,5.0% (12/240) ,31.3% (75/240) ,44.2% ( 106/ 240 ) ], P = 0. 026, 0. 008,0. 000,0. 001, OR = 0. 481,0. 157,0. 312,0.190. Multi -variate logistic regression demonstrated that the 95% CI of OR for each allele was 1. 010 - 2. 245,0. 757 - 1. 116,0. 603 - 1. 054, 0. 855 -1.014, 0.971 - 1.010, respectively. Conclusion The allele HLA-DR2 (15) is correlated with the susceptibility to childhood asthma whereas it is not related with the severity of asthma.
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