泡膜蛋白1在非小细胞肺癌组织中的表达及其预后价值
Expression and prognostic value of vacuole membrane protein 1 in non-small-cell lung cancer
目的 探讨泡膜蛋白1(VMP1)在非小细胞肺癌(NSCLC)中的表达及其预后价值.方法 收集NSCLC组织蜡块78例并进行随访,订制兔抗人VMP1抗体并通过免疫组化检测VMP1蛋白的表达,分析其与临床病理指标、术后生存率的关系.结果VMP1只表达于肿瘤细胞且定位于胞质,阳性率为35.9%(28/78),VMP1表达与临床分期关系密切,分期越晚,阳性率越高(x2=6.829,P<0.05),但与性别、年龄、组织类型、大体类型和分化程度等均无关(P>0.05).患者术后1年和3年的累积生存率分别为94.9%和66.3%,平均生存期为37.2个月(95% CI:33.7 ~40.8).VMP1表达对生存曲线分布有影响,其中阳性者预后较差(x2 =6.192,P<0.05),平均生存期仅为29.0个月(95%CI:25.0~33.0);VMP1表达和临床分期是影响患者术后生存的危险因素(P<0.05).结论 NSCLC组织中VMP1的表达具有较好的预后价值,VMP1阳性提示预后不良.
更多Objective To investigate the expression and prognostic value of vacuole membrane protein 1(VMP1)in non-small-cell lung cancer(NSCLC).Methods The clinical data and survival status of 78 NSCLC patients were collected.Their paraffin-embedded tissues were detected immunohistochemically with a custom-made rabbit anti-human VMP1 polyclonal antibody.And the clinical significance of VMP1 expression and its relationship with the overall survival rate were analyzed statistically.Results The positive expression of VMP1 could only be observed in cytoplasm of cancer cells.Among 78 patients,40 (51.3%)patients were stained VMP1-positive.The positive rate of VMP1 had a correlation with clinical stage(x2 =6.829,P < 0.05),but not with gender,age,pathological type,gross classification and differentiation grade(P > 0.05).The overall 1-and 3-year survival rate was 94.9% and 66.3%respectively.The overall estimated survival time was 37.2 months(95% CI:33.7-40.8).The expression of VMP1 had significant influence on the survival rate(x2 =6.192,P <0.05).The VMPl-positive patients lived shorter with an estimated survival time of 29.0 months(95 % CI:25.0-33.0).VMP1 expression and clinical stage were independent risk factors of influencing the survival rate.Conclusion The detection of VMP1 in resected NSCLC tumor tissues may be helpful for prognostic prediction.NSCLC patients with VMP1-positive or late clinical stage have a worse prognosis.
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