CHK1基因对白念珠菌生物特性的影响
Effects of histidine kinase gene CHK1 on some biological characteristics of Candida albicans
目的 研究白念珠菌组氨酸激酶基因CHK1基因对白念珠菌某些生物特性的影响.方法 观察CHK1基因突变菌株CHK21、CHK25、CHK26、CHK27的增殖、厚壁孢子形成、芽管形成能力和对刚果红耐受的情况.结果 CHK1基因突变株CHK25、CHK26、CHK27、CHK21的增生能力均低于标准株(6 h:1.36±0.86、1.25 ±0.84、1.05 ±0.79、0.90±0.74比1.54±0.89,P=0.000),CHK21菌株最为明显.CHK1基因突变菌株CHK21、CHK25、CHK26的芽管形成率均低于标准菌株(2 h:5.6%±2.0%、19.5%±6.9%、13.6%±4.8%比29.6%±10.5%,P=0.023、0.028、0.029).在无光线下,标准株和CHK26突变株在14 d形成的厚壁孢子数平均是为3和22个,有光线下分别为60和80个,CHK26形成厚壁孢子的能力比标准株强.无光线下CHK21株无厚壁孢子形成.CHK1基因突变株对刚果红敏感.结论 CHK1基因影响白念珠菌的增殖、厚壁孢子、菌丝的形成及对部分环境压力的耐受.
更多Objective To explore the effects of histidine kinase gene CHK1 on some biological characteristics of Candida albicans.Methods The effects of gene mutation strains of Candida albicans such as CHK21,CHK25,CHK26 and CHK27 were observed on its reproductive ability,formation of chlamydospore and germ tube and tolerance of Congo red.Results The reproductive ability in CHK gene mutation strains CHK25,CHK26,CHK27,CHK21 was weaker than that of wild strains(6 h:1.36 ±0.86,1.25 ± 0.84,1.05 ± 0.79,0.90 ± 0.74 vs 1.54 ± 0.89,P =0.000).And CHK21 was the most obvious.The formation of germ tube in CHK gene mutation strains CHK21,CHK25,CHK26 and CHK27 was weaker than that of wild strains (2 h:5.6% ± 2.0%,19.5% ± 6.9%,13.6% ± 4.8% vs 29.6% ± 10.5%,P =0.023,0.028,0.029).Under no light,the mean number of chlamydospore in wild and CHK26 strains was 3 and 22 respectively.With light,the mean number was changed to 60 and 80.So the formation ability of chlamydospore in CHK26 was stronger than other strains.CHK21 could not produce chlamydospore under no light.The mutation strain of CHK1 was sensitive to Congo red.Conclusion CHK1 affect the reproduction and formation of chlamydospore and hypha and the tolerance to some environmental pressures of Candida albicars.
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