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目的 观察NVM-1(Novel metastasis-promoting gene 1)蛋白在肝细胞癌(HCC)中的表达情况及意义.方法 应用免疫组化SP法检测92例HCC癌组织及非癌组织中NVM-1蛋白的表达水平,并分析其与HCC临床病理因素的相关性.结果 NVM-1蛋白主要定位在癌组织的细胞核,NVM-1蛋白在癌组织中阳性表达率(52.2％)高于非癌组织(28.3％)及肝脏良性病变的肝脏组织(0),差异有统计学意义(P＜0.05).NVM-1蛋白的表达与肿瘤的TNM分期(P=0.016)、BCLC分期(P=0.015)、病理分化程度(P =0.000)、肝内转移(P =0.002)、门脉癌栓(P=0.023)、复发(P=0.001)显著相关,但与HBV、肿瘤大小、个数、肝硬化等无相关性;Kaplan-Meier分析显示癌组织NVM-1表达阳性组中位无瘤生存时间为5个月,NVM-1表达阴性组中位无瘤生存时间为13个月,两者差异有统计学意义(P=0.001).结论 HCC癌组织中NVM-1的阳性表达可能与肿瘤的转移复发有密切关系.

Objective To explore the expression of NVM-1 (novel metastasis-promoting gene 1) in hepatocellular carcinoma (HCC) tissue and examine its clinical significance.Methods The expression of NVM-1 in 92 cases of HCC tumor tissues and non-tumor tissues was detected by immunohistochemical staining with SP (streptavidin-perosidase).And its clinical significance was evaluated.Results The staining of NVM-1 was predominantly located in the nucleus of HCC tumor tissue.The positive rate of NVM-1 was 52.2％ in all detected cancers and it was higher than that in control non-tumor tissues(28.3％) and benign hepatic lesions (0).A significant difference of positive rates existed between tumor and nontumor tissues (P ＜ 0.05).The expression of NVM-1 in HCC was not correlated with hepatic B virus (HBV),size,amount or cirrhosis,etc.However,it was correlated with TNM (P =0.016),BCLC stage (P =0.015),poor differentiation (P =0.000),intrahepatic metastasis (P =0.002),portal vein tumor thrombus (P =0.023) and recurrence (P =0.001).By Kaplan-Meier analysis,the median tumor-free survival time was 5 months in positive expression group and it was significantly shorter than 13 months in negative expression group (P =0.001).Conclusion There is probably a close relation between recurrence and metastasis and the expression of NVM-1 in HCC.