高脂血症新西兰大白兔慢性应激模型的建立及评价
Chronic stress model in New Zealand white rabbit with hyperlipidemia
目的 建立并评价高脂血症新西兰大白兔慢性应激(CS)模型.方法 45只雄性清洁级新西兰大白兔,随机数字表法分为:正常饮食组(CON)、正常饮食+慢性应激组(CON+ CS)、高脂饮食组(HFD)和高脂饮食+慢性应激组(HFD+ CS).HFD+ CS组高脂饮食4周后,叠加应激8周.应激方案采用社会应激联合躯体应激.组间比较用方差分析.结果 (1)模型评价:①体质量,应激后增长幅度减小;②行为学分析,CON+ CS、HFD+ CS组12周时不动行为[54%±7%,55%±5%]明显多于CON、HFD组[27%±5%,34%±6%,P<0.01,P<0.05];③血清学指标,8周时,HFD+ CS组比HFD组[(60 ±5) ng/ml比(38 ±4) ng/ml,P<0.01]皮质醇水平升高;12周时,血清超敏C反应蛋白(hs-CRP)和白细胞介素-6(IL-6)升高.(2)高脂对慢性应激的影响,HFD+ CS比CON+ CS组,不动行为增多,皮质醇,hs-CRP和IL-6升高.(3)血脂,应激后总胆固醇升高.结论 (1)高脂饮食4周基础上,采用社会应激联合躯体应激8周,可以成功建立高脂血症新西兰大白兔慢性应激模型;(2)高脂血症与慢性应激相互影响.
更多Objective To establish and evaluate chronic stress model in New Zealand white rabbit with hyperlipidemia.Methods A total of 45 clearing grade male New Zealand white rabbits were divided into four groups with random number table method:control (CON),normal diet combined with chronic stress for 8 weeks (CON + CS),high fat diet (HFD) and high fat diet for 4 weeks combined with chronic stress for 8 weeks (HFD + CS).Both social stress and physical stress methods were adopted.One-way ANOVA was used for comparison among groups.Results (1) Chronic stress model assessments:①body weight,the weight gain of stress groups was significantly reduced;②behavioral assessment,rabbits exposed to stress in CON + CS and HFD + CS group [54% ±7%,55% ±5%] exhibited more inactivity behavior than CON and HFD group [27% ± 5.28%,34% ± 6%,P < 0.01,P < 0.05];③serum indexes:after stress regime for 4 weeks,cortisol of HFD + CS was higher than HFD group [(60 ± 5) ng/ml vs (38 ± 4) ng/ml,P=0.001].Mter 8 weeks,the serum levels of hs-CRP and IL-6 also elevated.(2) The effect of hyperlipidemia on chronic stress:compared with CON + CS,HFD + CS group showed more inactivity behavior and rising levels of cortisol,hs-CRP and IL-6.(3) Blood lipids:chronic stress induced raised serum total cholesterol.Conclusions (1)Chronic stress model in rabbit with hyperlipidemia could be successfully established with 4-week high lipid feed followed by social stress combined with physical stress for 8 weeks.(2) Hyperlipidemia and chronic stress influences each other.
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