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目的 探讨配对盒基因8(Pax8)和配对盒基因2(Pax2)蛋白在不同类型的上皮及肿瘤组织中的表达差异和临床意义. 方法 采用组织芯片和免疫组化染色方法研究Pax8和Pax2蛋白在255例肿瘤组织和75例正常上皮组织中的表达差异. 结果 Pax2和Pax8蛋白在不同系统正常上皮组织中的表达呈现出一定的特异性.Pax8蛋白集中表达于甲状腺、泌尿系统及女性生殖系统的上皮组织,其阳性表达率分别为100%(2/2)、60.0%(3/5)和76.9%(10/13).Pax2蛋白集中表达于泌尿系统和女性生殖系统的上皮组织,其阳性表达率分别为40.0%(2/5)和38.5%(5/13).Pax2蛋白在正常甲状腺组织中不表达.Pax8蛋白在女性生殖系统中的阳性表达率高于Pax2蛋白(P<0.05).Pax2和Pax8蛋白在不同系统肿瘤组织中的表达呈现出一定的特异性.Pax8蛋白在甲状腺癌组织中的阳性表达率为66.7%(10/15),在肾细胞癌组织中的阳性表达率为65.2%(15/23),在子宫内膜腺癌组织中的阳性表达率为80.0%(4/5).Pax2蛋白的表达相对较弱,在甲状腺癌组织中的阳性表达率为13.3%(2/15),在肾细胞癌组织中的阳性表达率为34.8%(8/23),在子宫内膜腺癌组织中的阳性表达率为20.0%(1/5).Pax8蛋白在甲状腺癌、肾细胞癌和子宫内膜腺癌组织中的阳性表达率均明显高于Pax2蛋白在相关癌组织中的阳性表达率(均P<0.05).Pax8蛋白在卵巢浆液性癌、卵巢宫内膜样癌、卵巢透明细胞癌组织中的阳性表达率分别为92.9%(26/28)、81.8%(9/11)和82.4%(14/17),Pax2蛋白在卵巢浆液性癌、卵巢宫内膜样癌、卵巢透明细胞癌组织中的阳性表达率分别为28.6%(8/28)、9.1%(1/11)和17.6%(3/17),Pax8蛋白在卵巢浆液性癌、卵巢宫内膜样癌、卵巢透明细胞癌组织中的表达明显高于Pax2蛋白在相关癌组织中的表达(均P<0.05). 结论 Pax2和Pax8蛋白均可特异性表达于女性生殖道上皮和泌尿系统上皮,但Pax8蛋白的阳性表达率明显优于Pax2蛋白,二者联合使用对于不同器官来源的上皮性肿瘤的鉴别具有一定的临床意义.

Objective To explore the expressional differences between paired box genes 2(Pax2) and 8 (Pax8) protein in different kinds of epitheliums and tumors, and to investigate the clinicopathologic significance.Methods Expression levels of Pax2 and Pax8 protein were detected in 75 cases of different human epithelium tissues and 255 cases of different tumors on tissue microarray by immunohistochemistry. Results Pax2 and Pax8 selectively expressed in different tissues.The positive rates of Pax8 protein expressed in the normal epithelium of the thyroid, urinary system and female reproductive system were 100% (2/2), 60.0% (3/5) and 76.9% (10/13), respectively.The positive rates of Pax2 expressed in the epithelium tissues of urinary system and the female reproductive system were 40.0% (2/5) and 38.5% (5/13) respectively.However, the expression of Pax2 protein was not detected in the normal thyroid epithelium.The positive rate of Pax8 protein expressing in the epithelium of reproductive system was significantly higher than that of Pax2 protein (P<0.05).The tumors derived from different tissues also expressed different levels of protein Pax2 and Pax8.The positive rates of Pax8 in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were 65.2% (15/23), 66.7% (10/15) and 80.0% (4/5), respectively.The positive rates of Pax2 in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were 34.8% (8/23), 13.3% (2/15) and 20.0% (1/5), respectively.The positive rates of Pax8 protein expressed in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were significantly higher than those of Pax2 protein (P<0.05).The positive rates of Pax8 in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinoma were 92.9% (26/28), 81.8% (9/11) and 82.4% (14/17), respectively.The positive rates of Pax2 in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinoma were 28.6% (8/28), 9.1% (1/11) and 17.6% (3/17), respectively.The positive rates of Pax8 protein expressed in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinomawere significantly higher than those of Pax2 protein (P<0.05).Conclusions Pax2 and Pax8 are specifically expressed in female reproductive system and uritany system.However, the positive expression of Pax8 is superior to that of Pax2.The combined expression of Pax8 and Pax2 can be used in the differential diagnosis of epithelial tumors derived from different origins.