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目的 检测非小细胞肺癌细胞株中白细胞介素11(IL-11)及IL-11受体α(IL-11Rα)的表达情况,并探讨其临床意义.方法 采用蛋白印迹法检测非小细胞肺癌细胞株A549、H2228及正常肺小气道上皮细胞株(SAEC)细胞质、细胞膜及细胞核中IL-11和IL-11Rα的表达水平.结果 IL-11与IL-11Rα在SAEC细胞膜、细胞核中表达水平低(细胞膜:IL-110.04±0.03,IL-11Rα0.05±0.03;细胞核:IL-110.45±0.19,IL-11Rα0.07±0.02;均P<0.01).与SAEC相比,IL-11与IL-11Rα在A549及H2228细胞膜、细胞核中表达明显升高(P<0.01).A549细胞株中,IL-11及IL-11Rα在细胞核中的表达水平明显高于在细胞质与细胞膜中的表达(P<0.01),而H2228细胞株中,IL-11在细胞质中的表达水平最高(P<0.01),IL-11Rα在细胞核中的表达水平最高(P<0.01).结论 IL-11及IL-11Rα在非小细胞肺癌细胞株中呈现高表达,检测IL-11及IL-11Rα表达水平有助于肺癌的筛查及早期诊断.

Objective To detect the expressions of interleukin-11 (IL-11) and interleukin-11 receptorα(IL-11Rα) in non-small cell lung cancer (NSCLC) cell lines, and explore their clinical significances. Methods The expressions of IL-11 and IL-11Rαin NSCLC cell lines A549, H2228, healthy lung small airway epithelial cell (SAEC) line cytoplasm, cell membrane and nucleus were detected by Western blot. Results The expressions of IL-11 and IL-11Rα were low in the cell membrance and nucleus (cell membrane: IL-110.04± 0.03, IL-11Rα0.05±0.03; nuclear: IL-110.45±0.19, IL-11Rα0.07±0.02;P<0.01); The expressions of IL-11 and IL-11Rα in A549 and H2228 cell lines were significantly increased compared with those of SAEC cell lines in the cell membrance and cytoplasm (P< 0.01); Among the A549 cell lines, the expressions of IL-11 and IL-11Rα in cell nucleus were much higher than those of the cell membrance and cytoplasm (P< 0.01). Among the H2228 cell lines, the expression of IL-11 in cytoplasm was the highest and the expression of IL-11Rα was the highest in the cell nucleus (P< 0.01). Conclusion The expressions of IL-11 and IL-11Rαare high in NSCLC cell lines, and it is good for the screening and early diagnosis of lung cancer by detecting the expressions of IL-11 and IL-11Rα.