摘要目的 探讨高压氧治疗对阿尔茨海默病(Alzheimer disease,AD)小鼠脑组织中Th17/Treg细胞浸润的影响及其作用机制.方法 APP/PS1双重转基因小鼠给予高压氧治疗后免疫荧光染色和Western blotting检测脑组织中Aβ的沉积,流式细胞术检测脑组织中Th17细胞与Treg细胞的浸润情况.结果 高压氧治疗后的AD小鼠脑组织中Aβ沉积显著减少,差异有统计学意义(P＜0.01);另外,高压氧治疗使AD小鼠脑组织中IL-17升高、而FOXP3的表达却显著下降,差异均有统计学意义(均P＜0.01);高压氧治疗可上调AD小鼠脑组织中的Th17/Treg细胞比例(P＜0.01).结论 高压氧治疗可以通过上调AD小鼠脑组织中的Th17/Treg细胞比例,减低AD小鼠脑组织中Aβ的沉积,为高压氧治疗AD提供了实验依据.

abstractsObjective To explore the effects of hyperbaric oxygen (HBO) on Th17/Treg cell infiltration in the brain tissue of Alzheimer disease (AD) mice,and also to identify possible mechanism involved for the improvement of AD conditions.Methods Following HBO treatment of APP/PS1 double transgenic mice,immunofluorescence staining and Western blotting were used to detect the deposition of Aβ in the brain tissue.Flow cytometry was used to analyze the infiltration of Th17 cells and Treg cells in the brain tissue.Results The deposition of Aβ in the brain tissue of AD mice after HBO therapy was significantly reduced (P ＜ 0.01).In addition,HBO would increase the expression of IL-17,while it could significantly decrease the expression of FOXP3 (P ＜ 0.01).Flow cytometry detection results indicated that HBO therapy could up-regulate the proportion of Th17/Treg cells in the brain tissue of AD mice (P ＜ 0.01).Conclusion HBO could decrease Aβ deposition in the brain tissue through up-regulation of the proportions of Thl7/Treg cells in the brain tissue of AD mice.