• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
高级检索

检索历史 清除

[Gene-gene interaction on central obesity in school-aged children in China].

摘要:

<b>Objective:</b> To investigate possible effect of 6 obesity-associated SNPs in contribution to central obesity and examine whether there is an interaction in the 6 SNPs in the cause of central obesity in school-aged children in China. <b>Methods:</b> A total of 3 502 school-aged children who were included in Beijing Child and Adolescent Metabolic Syndrome (BCAMS) Study were selected, and based on the age and sex specific waist circumference (WC) standards in the BCAMS study, 1 196 central obese cases and 2 306 controls were identified. Genomic DNA was extracted from peripheral blood white cells using the salt fractionation method. A total of 6 single nucleotide polymorphisms (<i>FTO</i> rs9939609, <i>MC4R</i> rs17782313, <i>BDNF</i> rs6265, <i>PCSK1</i> rs6235, <i>SH2B1</i> rs4788102, and <i>CSK</i> rs1378942) were genotyped by TaqMan allelic discrimination assays with the GeneAmp 7900 sequence detection system (Applied Biosystems, Foster City, CA, USA). Logistic regression model was used to investigate the association between 6 SNPs and central obesity. Gene-gene interactions among 6 polymorphic loci were analyzed by using the Generalized Multifactor Dimensionality Reduction (GMDR) method, and then logistic regression model was constructed to confirm the best combination of loci identified in the GMDR. <b>Results:</b> After adjusting gender, age, Tanner stage, physical activity and family history of obesity, the <i>FTO</i> rs9939609-A, <i>MC4R</i> rs17782313-C and <i>BDNF</i> rs6265-G alleles were associated with central obesity under additive genetic model (<i>OR</i>=1.24, 95<i>%CI</i>: 1.06-1.45, <i>P</i>=0.008; <i>OR</i>=1.26, 95<i>%CI</i>: 1.11-1.43, <i>P</i>=2.98×10(-4); <i>OR</i>=1.18, 95<i>% CI</i>: 1.06-1.32, <i>P</i>=0.003). GMDR analysis showed a significant gene-gene interaction between <i>MC4R</i> rs17782313 and <i>BDNF</i> rs6265 (<i>P</i>=0.001). The best two-locus combination showed the cross-validation consistency of 10/10 and testing accuracy of 0.539. This interaction showed the maximum consistency and minimum prediction error among all gene-gene interaction models evaluated. Moreover, the combination of <i>MC4R</i> rs17782313-C and <i>BDNF</i> rs6265-G was associated with an increased risk of central obesity after adjustment for gender, age, Tanner stage, physical activity and family history of obesity. <b>Conclusions:</b> Our study showed that <i>FTO</i> rs9939609-A, <i>MC4R</i> rs17782313-C and <i>BDNF</i> rs6265-G alleles were associated with central obesity, and statistical interaction between <i>MC4R</i> rs17782313-C and <i>BDNF</i> rs6265-G increased risk of central obesity in school-aged children in China.

更多
作者: L W,Fu [1] ; M X,Zhang ; L J,Wu ; L W,Gao ; J,Mi
作者单位: Department of Epidemiology, Capital Institute of Pediatrics, Beijing 100020, China. [1]
期刊:
《Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi》 2017年38卷7期883-888页
DOI: 10.3760/cma.j.issn.0254-6450.2017.07.007
PMID: 28738459
发布时间: 2019-03-01
翻译满意度评价:
提交
  • 浏览:42

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

充值 订阅 收藏 移动端 使用
帮助