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流式细胞术在非霍奇金淋巴瘤诊断与分型中的应用

The value of flow cytometry in diagnosis and subclassification of non-Hodgldn lymphoma

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目的 探讨流式细胞术对非霍奇金淋巴瘤(NHL)诊断与分型的临床应用价值.方法 收集临床怀疑淋巴瘤的患者手术切除的新鲜淋巴结59例,流式细胞术(FCM)检测异常单克隆淋巴细胞数量及其免疫表型,分析FCM诊断及分型的结果与病理组织学诊断的符合率.结果 28例病理确诊NHL的淋巴结标本中,FCM检测到有异常克隆性淋巴细胞的24例,异常淋巴细胞的比例为4.28%~89.10%,其中确诊B-NHL 23例,T-NHL 1例.FCM对NHL的诊断与病理组织学诊断的符合率为85.71%;FCM对B-NHL诊断的特异度为100%,敏感度为92%.在FCM确诊的24例NHL中,根据异常细胞表型进一步分型,与病理诊断均相符.结论 FCM能准确确定克隆性淋巴细胞,协助诊断NHL,并在B-NHL的分型和鉴别诊断中起重要作用.

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abstracts:

Objective To evaluate the utility of flow cytometry (FCM) in diagnosis and subclassification of non-Hodgkin lymphoma (NHL). Methods The samples of lymph nodes biopsy from 59 cases clinically suspected of NHL were detected by flow cytometry; and clonal lymphocytes and their immunophenotypes were identified analyzed. The concordance between the results of flow cytometry and histopathology was analyzed. Results Among the 59 cases, flow cytometry was able to identify aberrant clonal lymphocytes in 24 of 28 NHL cases identified by histopathology, the neoplastic lymphocytes ranged from 4.28 % to 89.10 %; 23 cases were diagnosed as B-NHL and 1 case was diagnosed as T-NHL. Compared with histopathology, the accuracy of FCM was 85.71% in diagnosis of NHL. The specificity and sensitivity of FCM was 100 % and 92% in diagnosis of B-NHL. The accuracy of flow cytometry immunophenotyping in classification of 24 cases of NHL was consistent with that of histopathology. Conclusion Flow cytometry could be an ancillary technique in diagnosis of NHL by identifying aberrant clonal lymphocytes, and enable identification of B-NHL subtype.

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