多索茶碱对支气管哮喘大鼠肺部基质金属蛋白酶-9的影响
Effect of theophylline on metalloprotease-9 airway of asthmatic mouse
目的 探讨多索茶碱对支气管哮喘(简称哮喘)大鼠肺部炎症的影响,以及在哮喘发病时基质金属蛋白酶-9在肺部沉积的影响.方法 ①免疫组织化学法检测基质金属蛋白酶-9在肺部沉积的情况;②肺组织学检查;③酶联免疫吸附实验检测外周血总IgE水平.结果 哮喘组支气管肺泡灌洗液中及血清中IgE的水平明显高于对照组(P<0.05);基质金属蛋白酶-9在肺部的沉积也明显多于对照组;而多索茶碱干预组IgE水平以及外周血总IgE水平明显低于哮喘组(P<0.05),肺组织病变减轻,基质金属蛋白酶-9的沉积也明显减少(P<0.05).结论 多索茶碱能减少基质金属蛋白酶-9在肺部的沉积,延缓气道重塑的发展.
更多Objective To investigate effect of theophylline on inflammatory airway of asthmatic mouse, and depositive effect of metalloprotease-9 when patient fall in asthma. Methods ①Detect matrix metalloproteinase-9 deposit in bellows(immunohistochemical method). ② Inspection of bellows histological anatomy.③Detect the contents IgE of peripheral blood (ELISA). Results The group of asthma's level of IgE in bronchoalveolar lavage fluid higher than control group ( P <0. 05). The deposit of matrix metalloproteinase-9 in asthma more than control group. However,the contents of level of IgE in group of theophylline intervention were lower than in asthma ( P < 0. 05). The deposit of matrix metalloproteinase-9 have decrease obviously (P < 0. 05). Conclusions Theophylline can lessen the deposit of matrix metalloproteinase-9 have decrease obviously ( P <0. 05).
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