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矽肺患者血管紧张素Ⅰ转换酶基因多态性与其血清内活力关系

Relationship between angiotensin Ⅰ converting enzyme gene polymorphism and serum angiotensin Ⅰconverting enzyme activity in patients with silicosis

摘要:

目的 探讨矽肺患者血管紧张素Ⅰ转换酶(ACE)基因第16内含子序列的插入(I)/缺失(D)多态性与血清ACE活力的关系.方法 收集2011年7月至2016年4月在煤炭总医院就诊的住院及门诊矽肺患者114例(病例组)和健康人群109名(对照组).采用酶联免疫吸附法检测2组血清ACE活力;采用聚合酶链反应-限制性片段长度多态性检测2组ACE基因第16内含子序列I/D多态性.结果 病例组和对照组ACE基因I/D多态性分布比较,差异无统计学意义.分别在病例组和对照组比较血清ACE活力在II、ID、DD 3个基因型间差异性,2组均有统计学意义(P值均<0.01).病例组基因型ID与DD间血清ACE活力比较,差异有统计学意义(P<0.01),而对照组基因型ID与DD间血清ACE活力比较,差异无统计学意义.病例组ACE基因II、ID、DD 3个基因型分别占38.6%(44例)、43.0%(49例)、18.4%(21例),其中ID基因型ACE活力最高,为37.24(22.96,43.90)U;DD次之,为24.76(20.02,29.80)U;II最低,为20.46(14.53,30.51)U.对照组ACE基因II、ID、DD 3个基因型分别占44.0%(48例)、44.0%(48例)、11.9%(13例),其中ID基因型ACE活力最高,为26.64(18.15,31.71)U;DD次之,为19.92(16.96,24.16)U;II最低,为13.48(10.54,16.69)U.携带II和ID基因型的病例组血清ACE活力明显高于携带相同基因型的对照组(U值分别为427.5、651.0,P值均<0.01).病例组ACE活力高于对照组(P<0.01).ACE基因多态性与肺功能无明显关系(OR=0.98,95%CI:0.91~1.07,P=0.941),与矽肺病分期无明显相关性(OR=0.91,95%CI:0.05~18.18,P=0.736).结论 ACE基因多态性影响矽肺患者血清ACE活力,推测矽肺发病可能与高ACE活力有关.

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abstracts:

Objective To investigate the relationship between the insertion/deletion polymorphism ( including three genotypes II ,ID and DD) in the 16th intron of angiotensin Ⅰconverting enzyme ( ACE) gene and the serum ACE activity in patients with silicosis .Methods 114 patients with silicosis in Meitan General Hospital and 109 healthy control subjects were collected from July 2011 to April 2016 .The serum ACE activity was measured by enzyme linked immunosorbent assay . The genotyping of ACE gene polymorphism was performed by polymerase chain reaction-restriction fragment length polymorphism in silicosis group and healthy control group .Results There was no significant difference in the genotype distribution of ACE gene polymorphism between silicosis group and healthy control group .The serum ACE activity among the three genotypes was significant difference in silicosis group and healthy control group ( all P <0 .01) .There was significant difference between ID and DD genotypes in silicosis group ( P <0 .01) ,whereas there was no difference between the same two genotypes in healthy control group .In silicosis group ,the percentage of the II ,ID ,DD genotypes was 38 .6% (44 cases) ,43 .0% (49 cases) and 18 .4% (21 cases) ,respectively .The serum ACE activity in the patients with ID genotype was the highest [37 .24 (22 .96 ,43 .90) U] ,the second was DD genotype [ 24 .76 (20 .02 ,29 .80) U] ,the lowest was II genotype [20 .46 (14 .53 ,30 .51) U] .On the other hand ,in healthy controls ,the percentage of the II ,ID , DD genotypes was 44 .0% (48 cases) ,44 .0% (48 cases) ,and 11 .9% (13 cases) ,respectively .The serum ACE activity was the highest in healthy controls with ID genotype [ 26 .64 (18 .15 ,31 .71) U] ,the second was DD genotype [19 .92 (16 .96 ,24 .16) U] ,the lowest was II genotype [13 .48 (10 .54 ,16 .69) U] .The serum ACE activity in the patients with ID and II genotypes was higher than that in the healthy controls with the same two genotypes ( U = 427 .5 ,651 .0 ,all P < 0 .01) .The serum ACE activity in silicosis group was significantly higher than that in healthy control group ( P <0 .01) .There was no correlation between ACE gene polymorphism and the lung function ( OR =0 .98 ,95% CI :0 .91-1 .07 , P =0 .941) . There was no correlation between ACE gene polymorphism and the stage of silicosis ( OR = 0 .91 , 95% CI :0 .05-18 .18 , P = 0 .736) .Conclusions ACE gene polymorphisms influences the serum ACE activity in patients with silicosis .It is speculated that the morbidity incidence of silicosis may be related to high ACE activity .

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