蛋白质与DNA相互作用检测的研究进展
Research progress in the detection of DNA and protein interaction
从细菌到哺乳动物细胞,蛋白质与DNA的相互作用对于所有活体生物均不可或缺.为了执行细胞的生长、分化、分裂以及在受到外界环境刺激时的调节功能,DNA编码的遗传信息必须被精确转录,这对生物的生长、发育与进化极为重要.使用生物技术操纵蛋白质和DNA的相互作用,可调节某些毒力基因的表达,从而有助于疾病的治疗.随着科学研究的深入,越来越多的科学技术被应用蛋白质与DNA相互作用的研究领域.对蛋白质与DNA相互作用研究中常用技术的原理、优缺点及应用进行综述,有助于科研人员在研究中选取适当的方法.
更多From bacteria to mammalian cells, protein-DNA interactions are indispensable for all living organisms. In order to perform cell growth, differentiation, division, and regulation of cell function when stimulated by the external environment, DNA-encoded genetic information must be accurately transcribed, which is extremely important for the growth, development, and evolution of organisms. Manipulating the interaction of protein and DNA through biotechnology can modulate the expression of certain virulence genes, so as to contributing to the treatment of diseases. With the deepening of scientific research, more and more scientific technologies are applied in the field of protein and DNA interaction research. In this paper, the principles, advantages, disadvantages and applications of commonly used techniques in the study of protein-DNA interactions were reviewed, which will help researchers evaluate and select appropriate methods in their researches.
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