检索历史 清除

目的 探讨肝细胞黏附分子(hepaCAM)蛋白与几种多药耐药蛋白在肾癌中的表达及其相互关系.方法 使用免疫组织化学法检测人肾癌组织中不同区域的hepaCAM蛋白、多药耐药相关蛋白(MRP)、P-糖蛋白(P-gp)、肺耐药蛋白(LRP)、拓扑异构酶Ⅱ(TOPOⅡ)的表达并分析其相互关系.结果 在肿瘤的周边区,hepaCAM蛋白与MRP、P-gp、LRP蛋白都呈现阳性表达;而在肿瘤的中心区域,hepaCAM蛋白与P-gp、LRP表达为阴性或弱阳性,而MRP、TOPOⅡ蛋白表达为阳性.与肿瘤周边组比较,MRP、TOPOⅡ在肿瘤中心区表达明显增强(31.23±5.67:23.89±4.56;45.66±2.34:5.23±0.66),差异有统计学意义(t=-6.20,P=0.00;t=-100.16,P=0.00),而hepaCAM、P-gp、LRP表达明显减弱(3.21±1.12:27.25±2.23;2.34±0.33:51.23±3.45;4.22±1.78:44.23±1.45),差异有统计学意义(t=60.87,P=0.00;t=90.35,P=0.00;t =107.18,P=0.00).相关分析结果显示hepaCAM蛋白在肾癌肿瘤中心组织的表达与MRP蛋白表达相关(r=0.94,P=0.01),而与P-gp、LRP、TOPOⅡ蛋白表达无关(r =0.22,P=0.44;r =0.14,P=0.80;r =0.34,P=0.07).结论 hepaCAM蛋白在肾癌中的表达可能与其耐药相关.

Objective To investigate the expression and relationship between hepatocyte cell adhesion molecule (hepaCAM) protein and some multidrug resistance proteins in renal carcinoma tissue.Methods Expressions of hepaCAM,multidrug resistance associated protein (MRP),P-glycoprotein (P-gp),lung resistance protein (LRP),and topoisomerase Ⅱ (TOPO Ⅱ) protein were detected by immunohistochemistry in different areas of human renal cell carcinoma tissues and their relationships were analyzed.Results In the peripheral zone of renal tumor,hepaCAM,MRP,P-gp and LRP protein were showed positive expression.In the central region of the renal tumor,the expressions of hepaCAM,P-gp and LRP were negative or weakly positive,while the expressions of MRP and TOPO Ⅱ protein were positive.The expressions of MRP and TOPO Ⅱ protein in the central region of tumor were stronger than those in the peripheral zone of tunor (31.23 ±5.67 vs.23.89 ±4.56;45.66 ±2.34 vs.5.23 ±0.66),with statistically significant differences (t =-6.20,P =0.00;t =-100.16,P =0.00).While the expressions of other proteins (hepaCAM,P-gp and LRP) in the central region of tumor were weaker than those in the peripheral zone of tumor (3.21 ±1.12 vs.27.25±2.23;2.34±0.33 vs.51.23±3.45;4.22±1.78 vs.44.23 ± 1.45),with statistically significant differences (t =60.87,P =0.00;t =90.35,P =0.00;t =107.18,P =0.00).Correlation analysis showed that the expression of hepaCAM protein in the central region of renal carcinoma was related with the expression of MRP protein (r =0.94,P =0.01),but it was not related with the expressions of P-gp,LRP and TOPO Ⅱ protein (r=0.22,P=0.44;r=0.14,P=0.80;r=0.34,P=0.07).Conclusion The expression of hepaCAM protein in renal carcinoma may be related to tumor drug-resistance.