再生障碍性贫血模型小鼠骨髓造血细胞CD95和Bcl-2的表达
Expressions of CD95 and Bcl-2 in bone marrow haemopoietic cells from mice with aplastic anemia
目的 探讨再生障碍性贫血模型小鼠骨髓造血细胞凋亡过程中CD95和Bcl-2的表达以及相互之间的关系.方法 用辐射和免疫介导的方法 制造小鼠的再生障碍性贫血模型.用流式细胞术分析模型组和正常对照组CD34+细胞和CD45+细胞,以及细胞表面CD95和胞内Bcl-2的表达.结果 模型组骨髓细胞中低表达CD45的CD34+细胞(CD34+CD45low细胞)比例低于对照组[(0.85±0.19)%比(1.21±0.28)%,P<0.05].有核细胞中,CD95在模型组中的表达比例明显高于对照组[(66.30±7.68)%比(42.78±15.12)%],在这2组CD34+CD45+细胞的表达分别为(69.21±9.36)%和(22.31±3.33)%,CD34+CD45low细胞的表达分别为(53.70±16.46)%和(7.68±4.42)%,均P<0.01.Bcl-2的表达比例仅在模型组CD34+CD45low细胞中增加,与对照组相比差异有统计学意义[(7.60±2.40)%比(0.70±0.64)%,P<0.05].对照组细胞的CD95与Bcl-2表达无相关,而模型组CD34+CD45low和CD34+CD45low细胞中,CD95与Bcl-2表达均呈负相关(r分别为-0.93和-0.81,P<0.05).结论 再生障碍性贫血模型小鼠中,骨髓中造血细胞的比例下降,有核细胞和CD34+细胞的CD95表达比例升高,凋亡明显,针对不同的凋亡指标进行相应调节,可能是有效治疗再生障碍性贫血的途径之一.
更多Objective To investigate the expressions of CD95 and Bcl- 2 in bone marrow haemopoietic cells from mice with aplastic anemia and their relationship. Methods The mouse model of aplastic anemia was established by irradiation and immuno-mediation. Flow cytometry was employed to analyze the CD34+ cells, CD45+ cells, as well as expressions of surface CD95 and intracellular Bcl-2. Results The ratio of CD34+ cells with low expresson of CD45 (CD34+ CD45low) cells in the bone marrow was significantly lower in model group than that in control group [(0.85 ± 0.19)% vs (1.21±0.28)%, P< 0.05]. The ratio of CD95 expression in model group was increased significantly on nucleated cells compared to control group [(66.30±7.68)% vs (42.78±15.12)%], on CD34+CD45+ cells [(69.21±9.36)% vs (22.31± 3.33)%], and on CD34+CD45low cells [(53.70±16.46)% vs (7.68±4.42)%], respectively, all P<0.01. The ratio of Bcl-2 expression appeared comparable on different cells except on CD34+CD45low cells, which was (7.60±2.40)% in the model group and (0.70±0.64)% in control group with significant difference (P<0.05). The expressions of CD95 was not correlated with Bcl-2 in control group, but CD95 was negatively correlated with Bcl-2 on CD34+CD45+ celIs (r=-0.93, P<0.05)and CD34+CD45low cells (r=-0.81, P<0.05) of model group. Conclusions Mice with aplastic anemia showed lower ratio of haemopoietic cells, higher expression ratio of CD95 on nucleated and CD34+ cells in bone marrow, and obvious apoptosis. Regulation targeting at different markers of apoptosis may be helpful for effective treatment of aplastic anemia.
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