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替米沙坦干预心房颤动模型猪的右心房心肌靶蛋白鉴定

Identification of target proteins expressed in right atrial myocardium of swine models with atrial fibrillation intervened by telmisartan

摘要:

目的 研究替米沙坦(TMST)对实验性心房颤动(AF)模型猪的影响,分离并鉴定右心房组织差异表达蛋白.方法 健康家猪18只完全随机法分为3组,每组6只:正常对照(NC)组右心房植入电极不起搏,房颤(AF)组右心房植入电极并快速起搏,替米沙坦(TMST)组右心房植入电极快速起搏并给予TMST干预.实验前及2周后测量各组实验猪心室收缩末期左、右心房面积(LAESA、RAESA).实验结束时进行右房组织Masson染色观察,双向蛋白质电泳分离各组右心房心肌差异表达蛋白质,采用基质辅助激光解吸电离-飞行时间-质谱技术鉴定靶蛋白.结果 AF组采用快速右心房起搏成功建立AF猪模型.与AF组比较,TMST干预可以减少AF诱发率和胶原纤维表达,并使LAESA和RAESA缩小[LAESA:(630.6±10.9)mm2比(744.3±29.9)mm2;RAESA:(553.4±13.7)mm2比(677.9±26.3)mm2,均P<0.05].双向蛋白质电泳及质谱分析鉴定出翻译起始因子5A、热休克蛋白27、NADH脱氢酶铁硫蛋白8在TMST组表达减少.结论 翻译起始因子5A、热休克蛋白27、NADH脱氢酶铁硫蛋白8可能是与TMST减低实验猪AF诱发率、抑制心房重构相关的靶蛋白.

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abstracts:

Objective To explore the effect of telmisartan (TMST) on experimental swine models with atrial fibrillation (AF) , and to isolate and identify the target proteins expressed in right atrial myocardium.Methods Eighteen healthy pigs were randomly divided into three groups (n=6 each). Electrodes were implanted in the swines of three groups, with no pacing in control (NC) group, rapid atrial pacing (RAP) in the experimental AF model (AF group) and RAP plus telmisartan (TMST) intervention in the TMST group. Left and right atrial end-systolic areas (LAESA, RAESA) were measured by ultrasonic cardiogram before and 2 weeks after experiment. At the end of study, the histological change of right atrium was studied with Masson staining, and differentially expressed proteins of right atrium were isolated by twodimensional polyacrylamide gel electrophoresis (2-DE) ,and target proteins were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS). Results The AF model was successfully established with RAP.Compared with AF group, the TMST group had less induction rate of AF and collagenous fiber, and significantly reduced LAESA and RAESA [LAESA: (630.6±10.9) mm2 vs(744.3±29.9)mm2; RAESA:(553.4±13.7) mm2 vs (677.9±26.3) mm2, all P<0.05]. Three proteins (eukaryotic translation initiation factor 5A, heat shock protein 27 and NADH dehydrogenase Fe-S protein 8)showed declined expressions in the right atrial myocardium of TMST group. Conclusion Eukaryotic translation initiation factor 5A, heat shock protein 27 and NADH dehydrogenase Fe-S protein 8 may be target proteins associated with reduced induction rate of AF and inhibition of atrial remodeling by TMST intervention.

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作者: 李飞 [1] 张卓琦 [2] 张超群 [2] 徐晤 [2] 王志荣 [2]
分类号: R318
栏目名称: 论著
DOI: 10.3760/cma.j.issn.1674-1927.2011.02.002
发布时间: 2011-09-08
基金项目:
国家自然科学基金 江苏省医学重点人才资助项目 江苏省333工程资助项目 徐州医学院研究生科技创新计划
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