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Functional recovery after rhesus monkey spinal cord injury by transplantation of bone marrow mesenchymal-stem cell-derived neurons

摘要:

Background The treatment of spinal cord injury is still a challenge. This study aimed at evaluating the therapeutical effectiveness of neurons derived form mesenchymal stem cells (MSCs) for spinal cord injury.Methods In this study, rhesus MSCs were isolated and induced by cryptotanshinone in vitro and then a process of RT-PCR was used to detect the expression of glutamic acid decarboxylase (GAD) gene. The induced MSCs were tagged with Hoechst 33342 and injected into the injury site of rhesus spinal cord made by the modified Allen method. Following that, behavior analysis was made after 1 week, 1 month, 2 months and 3 months. After 3 months, true blue chloride retrograde tracing study was also used to evaluate the re-establishment of axons pathway and the hematoxylin-eosin (HE) staining and immunohistochemistry were performed after the animals had been killed.Results In this study, the expression of mRNA of GAD gene could be found in the induced MSCs but not in primitive MSCs and immunohistochemistry could also confirm that rhesus MSCs could be induced and differentiated into neurons. Behavior analysis showed that the experimental animals restored the function of spinal cord up to grade 2-3 of Tarlov classification. Retrograde tracing study showed that true blue chollide could be found in the rostral thoracic spinal cords, red nucleus and sensory-motor cortex.Conclusions These results suggest that the transplantation is safe and effective.

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作者单位: Department of Pathophysiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China [1] Department of Microsurgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China [2] Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510080, China [3] Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China [4]
期刊: 《中华医学杂志(英文版)》2005年118卷18期 1533-1541页 SCISCIEMEDLINEISTICCSCDCABP
分类号: R6
发布时间: 2005-11-24
基金项目:
the National Nature Science Foundation of China the Key Subject of Guangdong Province Technological Plan
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