Effect of leptin on bone metabolism in rat model of traumatic brain injury and femoral fracture
Objective: To observe serum and callus leptin expression within the setting of fracture and traumatic brain injury (TBI).Methods: Atotal of 64 male SD rats were randomized equally into 4 groups: nonoperated group, TBI group, fraeture group, and fracture+TBI group. Rats were sacrificed at 2, 4, 8 and 12 weeks after fracture+TBI. Serum leptin was detected using radioimmunoassay, and callus formation was measured radiologically. Callus leptin was analyzed by immunohistochemistry.Results: Serum ieptin levels in the fracture group, TBI group and combined fracture+TBI group were all significantly increased compared with control group at the 2 week time-point (P＜0.05). Serum leptin in the combined fracture +TBI group was significantly higher than that in the fracture and TBI groups at 4 and 8 weeks after injury (P＜0. 05).The percentage of leptin-positive cells in the fracture+TBI callus and callus volume were significantly higher than those in the fracture-only group (P＜0.01).Conclusions: We demonstrated elevated leptin expression within healing bone especially in the first 8 weeks in a rat model of fracture and TBI. A close association exists between leptin levels and the degree of callus formation in fractures.
|作 者||WANG Lei；YUAN Ji-shan；ZHANG Hong-xi；DING Hua；TANG Xing-guo；WEI Yong-zhong|
|刊 名||中华创伤杂志（英文版） 2011年14卷01期 7-13页|
|英文期刊名||CHINESE JOURNAL OF TRAUMATOLOGY|
|关键词||Leptin Brain injuries Fractures,bone|
|基金项目||the Medical Science and Technology Development of Jiangsu Health Bureau(项目编号：H200512)|