摘要目的 探讨变应性鼻炎(allergic rhinitis,AR)患者外周血中γδ T细胞、调节性T细胞(regulatory T cells,Treg)、白细胞介素(interleukin,IL) 17及转化生长因子β1(transforming growth factor β1,TGF-β1)的表达及临床意义.方法 2012年3-7月,收集32例AR患者(AR组)和20例健康志愿者(对照组)的外周血,采用流式细胞术检测外周血中γδ T细胞和Treg细胞比例；酶联免疫吸附试验检测血清中IL-17、TGF-β1的水平,并分析它们之间的相关性.采用SPSS 16.0软件进行数据分析.结果 γδ T细胞百分比在AR组和对照组分别为(13.30±8.62)％、(5.18±1.86)％,差异有统计学意义(t =5.18,P＜0.01)；Treg细胞百分比分别为(1.75±0.56)％、(4.76±1.74)％,差异有统计学意义(t =7.46,P＜0.01).AR组血清中IL-17的表达水平为(668.55 ±45.15) pg/ml,高于对照组的(573.53±17.42) pg/ml,差异有统计学意义(t=8.97,P＜0.01)；AR组TGF-β1的表达水平为(0.34±0.04) pg/ml,低于对照组的(0.49 ±0.06) pg/ml,差异有统计学意义(t=9.51,P＜0.01).AR组患者外周血中γδ T细胞百分比与Treg细胞百分比呈负相关趋势(r=-0.561,P＜0.01),γδ T细胞百分比与IL-17呈正相关趋势(r=0.469,P＜0.01),γδ T细胞百分比与TGF-β1呈负相关趋势(r=-0.622,P＜0.01),Treg细胞百分比与TGF-β1呈正相关(r=0.738,P＜0.01),IL-17表达水平与Treg细胞百分比、TGF-β1无相关性(r值分别为-0.111、-0.196,P＞0.05).结论 AR患者外周血中γδ T细胞和Treg细胞之间存在免疫平衡失调,γδ T细胞可能参与了IL-17的分泌,这可能在AR的发病和发展过程中发挥重要作用.

abstractsObjective To investigate the expression of peripheral blood γδ T cells/CD4 + CD25 + regulatory T cells(Treg) and cytokines interleukin 17 (IL-17) and transforming growth factor β1 (TGF-β1) in patients with allergic rhinitis.Methods From March 2012 to July 2012,32 patients with allergic rhinitis (AR group) and 20 healthy control subjects (control group) were collected.The expression of peripheral blood γδ T cells/Treg cells were measured by flow cytometry and the levels of IL-17 and TGF-β1 were evaluated by ELISA.SPSS 16.0 software was used to analyze the data.Results The percentages of γδT cells in AR group were (13.30 ±8.62)％,which was significantly higher (t =5.18,P ＜0.01) than those in control group (5.18 ± 1.86)％.The percentages of Treg cells in AR group were (1.75 ±0.56)％,which were significantly lower (t =7.46,P ＜ 0.01) than those in control group (4.76 ± 1.74) ％.The IL-17 levels in AR group were (668.55 ±45.15) pg/ml,which were also significantly higher (t =8.97,P ＜ 0.01) than those in control group (573.53 ± 17.42) pg/ml.The TGF-β1 levels in AR group were (0.34 ±0.04) pg/ml,which were also significantly lower (t =9.51,P ＜0.01) than those in control group(0.49 ± 0.06) pg/ml.There was a negative correlation between the percentages of γδ T cells and Treg cells (r =-0.561,P ＜0.01).There was a negative correlation between the percentages of γδ T cells and TGF-β1 levels (r =-0.622,P ＜ 0.01).A positive correlation was shown between the percentages of βδ T cells and IL-17 levels in AR (r =0.469,P ＜ 0.01).A positive correlation was shown between the percentages of Treg cells and TGF-β1 levels in AR (r =0.738,P ＜0.01).There was no correlation between IL-17 levels and the percentages of Treg cells or TGF-β1 levels (r value was-0.111,-0.196,all P ＞ 0.05).Conclusion There are imbalances of γδ T and Treg cells in peripheral blood of patients with allergic rhinitis.γδ T cells may be the main cell to produce IL-17,which may play an important role in allergic rhinitis.