X射线照射对小鼠辅助性T细胞相关细胞因子的影响
Effects of X-irradiation on the T-helper cell related cytokines in mouse
目的 观察不同剂量X射线照射对小鼠辅助性T细胞相关细胞因子的影响.方法 100只BALB /c小鼠用随机数字表法分为4组,包括健康对照组和X射线照射组(分别予2、4和6 Gy照射),每组25只.于照射前、照射后7、14、21和28 d,对小鼠一般情况进行观察,对外周血进行白细胞计数,用蛋白芯片检测血清中辅助性T细胞相关细胞因子含量,并用ELISA确证细胞因子的浓度变化.结果 照射后,小鼠外周血白细胞14d降至最低,同一时间点的下降程度随照射剂量增加而增加(F=86.267,P<0.05).芯片法初筛提示,细胞因子IFN-IFTGF-β、IL-6、IL-17上调,IL-5、IL-23、TNF-α下调.ELISA检测可见,IL-17浓度随辐射剂量增高而明显增高,6 Gy组在7、14和21 d均明显高于对照(t=23.743、21.759和17.662,P<0.05);IFN-γ/IL-4浓度在2和4 Gy照射后无明显变化,6 Gy照射后在7、14、21和28 d均比健康对照组明显增高(t=8.335、9.982、6.990和3.074,P<0.05),呈先升高后降低的变化趋势,14 d达高峰.结论 低于致死剂量的X射线照射可使小鼠血清IFN-γ 、TGF-β、IL-6、IL-17细胞因子浓度升高,呈Thl细胞偏移.
更多Objective To study the effects of X-ray irradiation at different doses on the expressions of the T-helper cell related cytokines in mouse.Methods 100 BALB/c mice were randomly divided into four groups (25 mice in each) with 0,2,4 and 6 Gy X-ray irradiation.At 1,7,14,21 and 28 d after irradiation,the general situation of mice were observed,and the peripheral white blood cells were counted.The alterations of the serum helper T cell related cytokines were screened by protein chips and tested by ELISA.Results At 14 d after irradiation,the peripheral white blood cells of mice decreased to the lowest level.The number of peripheral white blood cells decrease along with the irradiation dose (Fdose =86.267,P <0.05).There were significant increases in the release of IFN-γ,TGF-β,L-6 and IL-17,but decreases in cytokine IL-5,IL-23 and TNF-α.ELISA analysis revealed that the increases of IL-17 correlated with the irradiation dose positively and the concentration of IL-17 in 6 Gy group were higher than control group at 1,7,14 and 21 d after irradiation (t =23.743,21.759 and 17.662,P < 0.05).The ratio of IFN-γ/IL-4 significantly higher than the control group at 1,7,14,21 and 28 d after irradiation (t =8.335,9.982,6.990 and 3.074,P < 0.05),and it approached to the highest level at 14 d and then began to decrease.Conclusions A low dose X-irradiation induces T-helper cell related cytokines IFN-γ and IL-17,and leads to Th1-biased immune responses in mice in vivo.
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