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目的 探讨高压氧预处理(hyperbaric oxygen preconditioning,HBOP)对大鼠脑缺血再灌注后脑组织中血红素氧合酶-1(heme oxygenase-1,HO-1)和间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)表达的影响.方法 将SD大鼠按照数字表法随机分成假手术组(n=7)、缺血再灌注组(n=8)和HBOP+缺血再灌注组(n=8).将HBOP组大鼠置于0.2 MPa、85％氧浓度环境下1 h/d,连续7 d.采用改良的Longa法构建大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型,缺血2 h后再灌注,再灌注结束24 h后取材进行TTC染色及qRT-PCR实验检测HO-1、ALK的表达水平.结果 HBOP能够显著地降低脑梗死体积比(P<0.01).此外,HO-1、ALK表达水平在缺血再灌注组较假手术组明显提高,而HBOP组HO-1和ALK表达水平明显降低,但较假手术组HO-1表达仍有明显的上升(P<0.05).结论 HBOP能明显降低缺血再灌注大鼠脑组织中HO-1和ALK的表达水平,对缺血再灌注导致的脑组织损伤起到保护作用.

Objective To study the effects of hyperbaric oxygen ( HBO ) preconditioning on the expression of heme oxygenase-1 ( HO-1 ) and anaplastic lymphoma kinase ( ALK ) in cerebral ischemia-reperfusion ( IR) rats.Methods The Sprague-Dawley male rats were randomly divided into 3 groups: the middle cerebral artery occlusion (MCAO) and reperfusion group (n=8), the sham surgical group (n=7) and the HBO preconditioning +MCAO and reperfusion group (n=8).The HBO preconditioning rats were exposed to hyperbaric environment at a pressure of 0.2 MPa with 85％ oxygen concentration , 1 hour a day, for a succession of 7 days.Modified Longa method was used to develop middle cerebral artery occlusion ( MCAO) model and reperfusion was performed 2 hours after ischemia .Twenty-fours after termination of reperfusion , the samples were collected to perform TTC staining , and RT-PCR was used to detect the expression levels of HO-1 and ALK.Results HBO preconditioning could significantly reduce the scope of cerebral infarction ( P <0.01).Additionally, the expression levels of HO-1 and ALK in the ischemia and reperfusion group were markedly increased , as compared with those of the sham surgical group , while the expression levels of HO-1 and ALK in the HBO group were considerably decreased , however , the expression level of HO-1 was obviously increased as compared with that of the sham surgical group (P<0.05).Conclusion HBO preconditioning could significantly decrease the down-regulation of HO-1 and ALK in the brain tissue of ischemia and reperfusion rats , which indicated that it might have a protective effect on the brain damage induced by ischemia and reperfusion .