脐带间质干细胞移植对MRL/lpr狼疮鼠间质性肺炎的影响
The effect of umbilical cord mesenchymal stem cell transplantation on interstitial pneumonitts it MRL/lpr mice
目的 探讨脐带间质干细胞(UC-MSCs)对MRL/lpr狼疮鼠间质性肺炎的治疗作用.方法 体外分离培养UC-MSCs,将24只18周龄雌性MRL/lpr小鼠采用随机数字表法分为1次治疗组、3次治疗组和对照组,1次治疗组和3次治疗组于第18周给予1×106第3代UC-MSCs尾静脉注射,3次治疗组在第19、20周时再分别重复1次;对照组给予0.5 ml生理盐水尾静脉注射.在第29周时处死小鼠,用HE染色观察各组小鼠的肺病理变化.结果 UC-MSCs移植能显著减轻MRL/lpr狼疮鼠的间质性肺炎.1次治疗组和3次治疗组的肺气管损害指数分别为1.40±0.24和1.02±0.29,明显低于对照组的1.95±0.35,差异有统计学意义(q值分别为0.551和0.937,均P<0.01);1次治疗组和3次治疗组的血管损害指数分别为1.20±0.18和1.08±0.16,明显低于对照组的1.56±0.32,差异有统计学意义(q值分别为0.360和0.479,P<0.05和P<0.01);1次治疗组和3次治疗组的肺间质中炎症细胞浸润程度分别为1.30±0.21和1.05±0.15,明显低于对照组的1.72±0.34,差异有统计学意义(q值分别为0.417和0.673,P<0.05和P<0.01).结论 UC-MSCs移植对MRL/lpr狼疮鼠的间质性肺炎具有治疗作用.
更多Objective To investigate the effects of umbilical cord mesenchymal stem cell (UC-MSCs) transplantation on interstitial pneumonitis in MRL/lpr mice. Methods Twenty four 18-week-old MRL/lpr female mice were randomly divided into 3 groups: the single dose group received a single dose of UC-MSCs (1×10<'6>) transfusion intravenously, the 3 dose group received 3 injections of UC-MSCs (1×10<'6>) weekly for 3 weeks, and the control mice were treated with saline. Both the control and the treated mice were sacrificed at 29 weeks. The histopathology of the lungs were assessed by HE staining. Results In comparison to the control mice, UC-MSCs transplantation significantly attenuated interstitial pneumonitis in the MRL/lpr mice. The peribronchiolar lesion indices of the single dose treatment group (1.40±0.24) and the 3 dose treatment group (1.02±0.29) were significantly decreased as compared to the control group (1.95±0.35), q=0.551,0.937, all P<0.01. The perivascular lesion index of the single dose treatment group (1.20±0.18) and the 3 dose treatment group (1.08±0.16) were also significantly reduced as compared to the control group (1.56±0.32), q =0.360, 0.479, P<0.05, P<0.01. The inflammatory cell infiltration index of the control group (1.72±0.34) was significantly increased compared to the single dose treatment group (1.30±0.21) and the 3 dose treatment group (1.05±0.15), q=0.417,0.673, P<0.05, P<0.01. Conclusions These findings indicate that UC-MSCs have a pleiotropic therapeutic effect on pneumonitis in MRL/lpr mice.
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