摘要目的 比较来自颞下颌关节盘穿孔与髁突肥大患者关节盘来源的关节盘细胞合成Ⅰ型胶原的差别,探讨颞下颌关节盘穿孔时关节盘细胞合成及分泌Ⅰ型胶原能力的改变及其在颞下颌关节盘穿孔发生中的作用.方法 收集武汉大学口腔医学院颞下颌关节髁突肥大(对照组)及颞下颌关节盘穿孔(穿孔组)患者因手术切除的手术标本,采用酶消化法原代培养,培养的细胞经甲苯胺蓝染色及免疫荧光染色鉴定为成纤维样细胞.第1代细胞培养48 h后,采用蛋白质印迹法检测关节盘细胞内Ⅰ型胶原蛋白的表达,应用酶联免疫吸附测定法检测培养上清液内Ⅰ型胶原蛋白的含量.结果来自穿孔组及对照组的颞下颌关节盘均可培养出成纤维样细胞,甲苯胺蓝染色、Ⅰ型胶原及Ⅱ型胶原免疫荧光染色均表达阳性.培养48 h后培养上清液内Ⅰ型胶原的含量对照组[(1.62±0.52)μg/L]显著高于关节盘穿孔组[(0.85±0.33)μg/L](P=0.0134).蛋白质印迹法检测关节盘穿孔组细胞内Ⅰ型胶原的蛋白表达低于对照组.结论 颞下颌关节盘穿孔时关节盘成纤维样细胞内表达及分泌的Ⅰ型胶原含量均较对照组降低,这可能与关节盘细胞外基质中Ⅰ型胶原含量减少,进而导致关节盘穿孔的形成有关.

abstractsObjective To investigate the effect of temporomandibular joint (TMJ) disc perforation on expression of typeⅠcollagen in TMJ disc cells. Methods The fibroblastic-like cells from the surgical removed TMJ disc tissue (disc perforation or TMJ condyle hyperplasia) were cultured. The cultured cells were identified as fibroblastic-like cells by toluidine blue and immunofluorescence staining. The expression of typeⅠcollagen was detected with Western blotting and the content of typeⅠcollagen was examined by enzyme linked immunosorbent assay (ELISA). Results Fibroblastic-like cells were cultured from TMJ disc cells and the controls. The collagen-Ⅰ and collagen-Ⅱ were positive in both toluidine blue and immunofluorescence staining. In Western blotting, the expression of typeⅠcollagen in cells from joints with disc perforation was lower than that from normal joints. The content of collagen-Ⅰ was (1.62 ± 0.52) μg/L from controls, and (0.85±0.33)μg/L from disc perforation respectively (P=0.0134). Conclusions The disc cells from TMJ with disc perforation expressed lower typeⅠcollagen than that from controls, which may be related to the lower content of collagen-Ⅰin TMJ disc and the formation of TMJ disc perforation.