• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
高级检索

检索历史 清除

酶联免疫斑点法在诊断艾滋病合并结核病中的应用研究

Enzyme-linked immunospot assay in diagnosis of Mycobacterium tuberculosis infection for AIDS patients

摘要:

目的 探讨T细胞酶联免疫斑点法(T SPOT)在我国用于诊断艾滋病合并结核病的应用价值.方法 应用T SPOT对35例明确诊断的艾滋病合并结核病患者的血液样本进行结核分枝杆菌特异性T细胞的检测,同时对所有患者做PPD试验进行比较.结果 在艾滋病合并结核病的所有患者、CD4<200/μl、CD4>200/μl、抗结核疗程<2个月及抗结核疗程>2个月的各组患者中,T SPOT检测的阳性检出率分别为65.71%、66.67%、60.00%、83.33%和25.00%,而PPD试验的阳性检出率仅为24.24%、25.00%、33.33%、9.09%和25.00%,其中在患者总体、CD4<200/μl组、抗结核疗程<2个月组中,T SPOT检测的阳性检出率远高于PPD试验,差异有统计学意义(P<0.05).结论 TSPOT检测的敏感性高于目前常用的PPD试验,适用于我国艾滋病合并结核病的快速诊断.

更多
abstracts:

Objective To evaluate the application of enzyme-linked immunospot assay (T SPOT) in diagnosis of Mycobacterium tuberculosis (MTB) infection for AIDS patients. Methods The rapid T SPOT assay was employed to detect ESAT-6 and CFP-10 specific T cells in blood samples from 35 AIDS patients with active tuberculosis. The results were compared with those of PPD skin test. Results The positive-MTB rate with T SPOT assay in all patients (n = 35 ) was 65.71%, that in patients with CD4 < 200/μ (n = 21) was 66.67%, in patients with CD4 > 200/μl (n = 10) was 60.0% , in patients with anti-tuberculosis treatment < 2 months ( n = 15 ) was 83.33% and in patients with treatment > 2 months ( n = 6 ) was 25.00%. The positive rates of PPD skin test were 24.24%, 25.00%, 33.33%, 9.09% and 25.00% in these groups, respectively. The positive rates of T SPOT assay were significantly higher than those of PPD skin test in all patients, in those with CD4 < 200/μl and with anti-tuberculosis treatment < 2 months ( P < 0.05 ). Conclusion T SPOT assay was more sensitive than conventional PPD skin test, and can be applied in the diagnosis of MTB infections for AIDS patients.

More
  • 浏览:422
  • 下载:164

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

扩展文献

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

充值 订阅 收藏 移动端 使用
帮助