检索历史 清除

目的 了解禽流感病毒(AIV)在感染小鼠前后表面蛋白编码基因的特点及变异情况.方法 在A/Goose/Guangdong/NH/2003(H5N1)感染小鼠肺组织的第12小时和第9天各分离1株AIV病毒,通过鸡胚增殖后提取RNA,反转录合成cDNA,经PCR扩增和产物纯化构建重组质粒,用双脱氧链终止法进行核苷酸序列测定并进行基因特性分析.结果 3株病毒HA基因的核苷酸序列同源性为99.6%～99.8%,推导氨基酸序列的同源性为99.3%～99.6%.3株病毒NA基因的核苷酸序列同源性为99.8%～99.9%,均为同义突变.M基因未发生任何变异.结论 AIV感染小鼠后HA基因出现变异,NA和M基因未出现有意义的突变.

Objective To investigate the variations of surface protein genes of avian influenza virus (AIV)before and after infecting mouse.Methods Mouse lung tissue was infected with A/Goose/Guangaong/NH/2003(H5N1)and the virus was isolated 12 hours and 9 days after replication in lung tissue of mouse.The isolated strains were amplified in embryonated chicken eggs,anti the virion RNA was transcribed into cDNA by reverse transeriptase.After amplification and purification,dideoxy-mediated chain termination was performed to detect synthetic oligonucleotide primers and DNA sequence was analyzed.Results The homology of nucleotide sequence for HA gene of three isolated strains was 99.6%-99.8%.and that of amino acid sequences was 99.3%-99.6%.The homology of nucleotide sequence for NA gene of three strains was 99.8%-99.9%.all of them were synonymous mutatinns.No variation was found in M gene.Conclusion After replication in mouse lung tissue,no significant mutation was found in the surface protein genes of AIV except some point mutations in HA genes.